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What we can do

  • Trypan Blue Cell Counting
  • Viability and GFP Transfection
  • Antibodies Affinity
Trypan Blue Cell Counting
Trypan Blue Cell Counting

Trypan Blue Cell Counting

Monitoring and analyzing cell culture with state-of-the-art solutions. Reliable and efficient monitoring is crucial for optimizing yield and product quality since even small changes in bioprocess parameters can influence the performance of your cell culture. Cell count and viability are the most important parameters, Countstar® Altair supplies an extremely smart and fully comply with cGMP solution for these.

Viability and GFP Transfection
Viability and GFP Transfection

During the bioprocess, GFP is often used to fuse with recombinant protein as an indicator. Determine the GFP fluorescent can reflect the target protein expression. Countstar Rigel offers a fast and simple assay for testing GFP transfection as well as viability. Cells were stained with Propidium iodide (PI) and Hoechst 33342 to define the dead cell population and total cell population. Countstar Rigel offers a quick, quantitative method for evaluating GFP expression efficiency and viability at the same time.

Antibodies Affinity
Antibodies Affinity
The affinity antibodies are usually measured by Elisa or Biacore, these methods are very sensitive, but they detect the antibody with the purified protein, but not natural conformation protein. Use cell immunofluorescence method, user can detect the antibody affinity with natural conformation protein. Currently, quantification of the affinity of antibody is analyzed by flow cytometry. Countstar Rigel also can provide a quick and easy way to evaluate the affinity of antibody.
Countstar Rigel can automatically capture the image and quantitative the fluorescence intensity which can reflect the antibody affinity.

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    Related Resources

    T/NK Cell Mediated Cytotoxicity Analysis by CFSE, Hoechest 33342 and PI

    Experimental Protocol       The cytotoxicity % is calculated by the equation below. Cytotoxicity % = (Live counts of control – Live counts of treated) / L…

    December 30, 2020

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