Kwayoyin dabbobi masu shayarwa ana amfani da su sosai a cikin Biopharmaceuticals, kamar ƙwayoyin rigakafi, rigakafi, peptides da metabolites na biyu ana samar da su ta hanyar bioprocessing tare da ƙwayoyin mammalian.A yayin duka tsari daga antibody R&D zuwa samarwa, akwai matakai da yawa da ake buƙata don aiwatar da gwajin tushen tantanin halitta don kimanta tsari ko sarrafa inganci.Kamar jimlar tattarawar tantanin halitta da iyawa za su ayyana matsayin al'adar tantanin halitta.Kazalika canjin tantanin halitta, alaƙar antibody tana ƙayyade a matakin tantanin halitta.Kayan aikin Countstar sune sitometry na tushen hoto, na iya taimakawa saka idanu daga R&D zuwa ayyukan samarwa da tabbatar da sake haifuwa da daidaito.
Ƙididdiga ta Tantanin halitta da Ƙarfafawa ta Ƙa'idar Tabbataccen Tambarin Trypan
Kulawa da nazarin al'adun tantanin halitta tare da mafita na zamani.Dogaro da ingantaccen saka idanu yana da mahimmanci don haɓaka yawan amfanin ƙasa da ingancin samfur tunda ko da ƙananan canje-canje a cikin sigogin bioprocess na iya rinjayar aikin al'adar tantanin ku.Ƙididdiga ta salula da iyawa sune mafi mahimmancin sigogi, Countstar Altair yana ba da wayo sosai kuma yana cika cikakken bayanin cGMP don waɗannan.
An ƙirƙira Countstar Altair bisa ƙa'idar keɓancewa ta Trypan Blue, tana haɗa babban benci na gani na gani na gani "gyara mayar da hankali", mafi kyawun fasahar gano tantanin halitta, da algorithms software.Ba da damar samun bayanan tattarawar tantanin halitta, yuwuwa, ƙimar tarawa, zagaye, da rarraba diamita ta hanyar gudu ɗaya.
Yiwuwa da Ƙaddamar Canjawar GFP a cikin Kwayoyin
A lokacin aikin bioprocess, GFP galibi ana amfani da shi don haɗa furotin da ke sake haɗawa azaman mai nuna alama.Ƙayyade mai kyalli na GFP na iya nuna maƙasudin furotin da aka yi niyya.Countstar Rigel yana ba da ƙima mai sauri da sauƙi don gwada canjin GFP har ma da yuwuwa.An lalata ƙwayoyin sel tare da Propidium iodide (PI) da Hoechst 33342 don ayyana matattun adadin tantanin halitta da jimillar yawan tantanin halitta.Countstar Rigel yana ba da hanya mai sauri, ƙididdigewa don kimanta ingancin magana da GFP a lokaci guda.
Sel suna samuwa ta amfani da Hoechst 33342 (blue) kuma ana iya ƙaddara yawan adadin GFP masu bayyanawa (kore) cikin sauƙi.Kwayoyin da ba za a iya amfani da su ba suna da tabo da propidium iodide (PI; ja).
Dangantakar gano antibody akan Countstar Rigel
An narkar da maganin rigakafi a cikin nau'i daban-daban, sa'an nan kuma a sanya shi tare da sel.An samo sakamakon daga Countstar Rigel (duka hotuna da sakamakon ƙididdiga)
Countstar ya shirya GMP don 21 CFR Part 11
Kayan aikin Countstar sun cika cikar 21 CFR da Sashe na 11, ayyukan IQ/OQ/PQ suna tabbatar da sarrafa daidaitaccen aiki.An shirya kayan aikin Countstar a cikin GMP da 21 CFR part 11 dakunan gwaje-gwaje masu dacewa.Ikon mai amfani da hanyoyin dubawa suna ba da damar isassun takaddun amfani tare da daidaitattun rahotannin PDF.
Takaddun IQ/OQ da sassan tabbatarwa
