Samfurin da ke ɗauke da sel a cikin dakatarwa yana haɗe tare da ruwan shuɗi na Trypan, sannan aka zana shi cikin Countstar Chamber Slide wanda Countstar Automated Cell Counter ya bincika.Dangane da ka'idar kirga tantanin halitta trypan blue cell, kayan aikin Countstar suna haɗa fasahar hoto mai ci gaba, fasahar gano hoto mai hankali da algorithms mai ƙarfi don ba wai kawai samar da tattarawar tantanin halitta da yuwuwa ba, har ma da samar da bayanan tattarawar tantanin halitta, yuwuwar, ƙimar tarawa, zagaye. , da kuma rarraba diamita tare da gudu ɗaya kawai.
Haɗaɗɗen Tantanin halitta
Hoto na 3 Kidayar sel da aka tara.
A. Hoton samfurin salula;
B. Hoton Samfurin Cell tare da alamar ganewa ta software na Countstar BioTech.(Green Circle: Live cell, Yellow Circle: Dead cell, Red Circle: Aggregated Cell).
C. Haɗaɗɗen Histogram
Wasu sel na farko ko ƙananan al'adu suna da wuyar haɗuwa lokacin da al'ada mara kyau ko kuma yawan narkewa, don haka suna haifar da matsala mai yawa a cikin ƙidayar sel.Tare da Aggregation Calibration Aiki, Countstar na iya gane ƙididdige ƙididdige ƙididdigewa don tabbatar da ingantacciyar ƙidayar tantanin halitta da kuma samun ƙimar tarawa da kuma lissafin tarawa, don haka yana ba da tushe ga masu gwaji don yin hukunci game da yanayin sel.
Kula da Girman Tantanin halitta
Hoto na 4 Hannun Girman Tantanin halitta.
Hannun haɓakar salula hanya ce ta gama gari don auna cikakkiyar ci gaban lambar tantanin halitta, alama ce mai mahimmanci don tantance tattarawar tantanin halitta kuma ɗayan mahimman sigogi na al'adar ainihin abubuwan halitta na sel.Domin a kwatanta daidaitaccen canji mai ƙarfi a cikin adadin ƙwayoyin sel a cikin duka tsari, ana iya raba madaidaicin haɓakar haɓaka zuwa sassa 4: lokacin shiryawa tare da jinkirin girma;Lokacin girma mai ma'ana tare da babban gangare, lokacin tudu da lokacin raguwa.Za a iya samun lanƙwan haɓakar tantanin halitta ta hanyar ƙirƙira adadin sel masu rai (10'000/mL) akan lokacin al'ada (h ko d).
Auna Tattaunawar Tantanin Halitta da Ƙarfafawa
Hoto na 1 Countstar BioTech ya ɗauki hoto azaman sel (Vero, 3T3, 549, B16, CHO, Hela, SF9, da MDCK) a cikin dakatarwar da Trypan Blue ta yi.
Countstar yana aiki ga sel masu diamita tsakanin 5-180um, kamar tantanin dabbobi masu shayarwa, tantanin kwari, da wasu planktons.
Ma'aunin Girman Tantanin halitta
Hoto 2 Ma'aunin Girman Tantanin halitta na ƙwayoyin CHO kafin da kuma bayan canjin plasmid.
A. Hotunan dakatarwar sel na CHO wanda aka zana ta hanyar blue trypan kafin da bayan canjin plasmid.
B. Kwatanta girman sel CHO kafin da kuma bayan canjin plasmid.
Canjin girman tantanin halitta shine maɓalli mai mahimmanci kuma ana auna shi a cikin binciken tantanin halitta.A al'ada za a auna shi a cikin waɗannan gwaje-gwajen: Canja wurin tantanin halitta, gwajin magani da ƙididdigar kunna tantanin halitta.Countstar yana ba da bayanan ƙididdiga masu ƙididdiga, kamar girman sel, cikin 20s.
Ƙa'idar tantanin halitta mai sarrafa kansa na Countstar na iya ba da bayanan halittar sel, gami da da'ira da diamita histogram.
