Takaitawa: Mesenchymal stem Kwayoyin wani yanki ne na sel masu ƙarfi masu ƙarfi waɗanda za a iya keɓance su daga mesoderm.Tare da sabuntawar sake maimaita kansu da halayen bambance-bambancen shugabanci da yawa, suna da babban damar yin amfani da magunguna daban-daban.Mesenchymal stem Kwayoyin suna da na musamman na rigakafi phenotype da ikon sarrafa rigakafi.Don haka, an riga an yi amfani da sel mai tushe na mesenchymal sosai a cikin dashen kwayoyin halitta, injiniyan nama, da dashen gabobin jiki.Kuma Bayan waɗannan aikace-aikacen, ana amfani da su azaman kayan aiki mai kyau a cikin injiniyan nama azaman ƙwayoyin iri a cikin jerin gwaje-gwajen bincike na asali da na asibiti.Har ya zuwa yanzu, babu wata hanyar da aka yarda da ita da kuma ma'auni don kula da ingancin sel masu kara kuzari.Countstar Rigel na iya sa ido kan maida hankali, iyawa, da halayen phenotype (da canje-canjen su) yayin samarwa da bambance-bambancen waɗannan sel masu tushe.Countstar Rigel kuma yana da fa'ida wajen samun ƙarin bayanan ilimin halittar jiki, wanda ingantaccen filin haske da rikodi na tushen haske ya samar yayin duk aikin sa ido kan ingancin tantanin halitta.Countstar Rigel yana ba da hanya mai sauri, ƙwaƙƙwalwa, kuma abin dogaro don ingantaccen sarrafa ƙwayoyin kara.
Kaya da matakai:
Abubuwan da aka samu na adipose mesenchymal stem cell (AdMSCs) sun sami baiwa ta Farfesa Nianmin Qi, maganin tabo AO/PI (Shanghai RuiYu, CF002).Antibody: CD29, CD34, CD45, CD56, CD73, CD105, HLADR (Kamfanin BD).
An haɓaka AdMSCs a cikin incubator mai humidified 37 ℃, 5% CO2.Narke tare da trypsin kafin amfani.
An bi hanyar tabon CD azaman jagorar antibody.
Gano alamar CD tare da Countstar Rigel:
1. An kirkiro hanyar aikace-aikacen siginar siginar ta hanyar saita tashar PE zuwa hoton PE fluorescence.
2. An kama filaye 3 daga kowane ɗaki.
3. Bayan hoton da bincike na farko sun cika, saitin ƙofar (ƙofa na log) don canzawa mai kyau da mara kyau an saita ta software ta FCS.
Ingantattun kula da kwayar halitta
Hoto na gaba (Figure 1) yana nuna hanyar maganin ƙwayar cuta .
Hoto 1: Hanya don maganin ƙwayar cuta
Sakamako:
Ƙayyadaddun tattarawa, iyawa, diamita, da tarawar AdMSCs.
Amincewar AdMSCs an ƙaddara ta AO/PI, An ƙirƙiri hanyar aikace-aikacen aikace-aikacen launi biyu ta hanyar saita tashar Green da tashar Red zuwa hoton AO da PI fluorescence, da filin haske.An nuna Hotunan Misali a Hoto na 2.
Hoto 2. Hotunan kafin jigilar kaya da kuma bayan jigilar AdMSCs.A. Kafin sufuri;an nuna hoton wakilci.B. Bayan sufuri;an nuna hoton wakilci.
An canza yuwuwar AdMSCs sosai bayan sufuri idan aka kwatanta da kafin jigilar kaya.Yiwuwa kafin jigilar kaya shine 92%, amma ya ragu zuwa 71% bayan sufuri.An nuna sakamakon a hoto na 3.
Hoto 3. Sakamakon yuwuwar AdMSCs (Kafin sufuri da bayan sufuri)
Countstar Rigel kuma ya ƙaddara diamita da tarawa.An canza diamita na AdMSCs sosai bayan sufuri idan aka kwatanta da kafin jigilar kaya.Diamita kafin jigilar kaya shine 19µm, amma ya karu zuwa 21µm bayan sufuri.Jimlar kafin jigilar kaya shine 20%, amma ya karu zuwa 25% bayan sufuri.Daga hotunan da Countstar Rigel ya ɗauka, an canza nau'in AdMSCs sosai bayan sufuri.An nuna sakamakon a hoto na 4.
Hoto 4: Sakamakon diamita da tarawa.A: Hotunan wakilan AdMSCs, nau'in AdMSCs an canza su sosai bayan sufuri.B: Tari kafin jigilar kaya shine 20%, amma ya karu zuwa 25% bayan sufuri.C: Diamita kafin jigilar kaya shine 19µm, amma ya karu zuwa 21µm bayan sufuri.
Ƙayyade immunophenotype na AdMSCs ta Countstar Rigel
Immunophenotype na AdMSCs an ƙaddara ta Countstar Rigel, AdMSCs an haɗa su da antibody daban-daban (CD29, CD34, CD45, CD56, CD73, CD105, HLA-DR).An ƙirƙiri hanyar aikace-aikacen launi na sigina ta hanyar saita tashar Green zuwa hoton PE fluorescence, da fili mai haske.An yi amfani da sashin tunani na hoto mai haske azaman abin rufe fuska don samfurin siginar walƙiya ta PE.An nuna sakamakon CD105 (Hoto na 5).
Hoto 5: Countstar Rigel ya ƙaddara sakamakon CD105 na AdMSCs.A: Ƙididdigar ƙididdiga na ƙimar ƙimar CD105 a cikin samfurori daban-daban ta FCS express 5 da software.B: Hotuna masu inganci suna ba da ƙarin bayani game da yanayin halitta.C: Ingantattun sakamako ta thumbnails na kowane tantanin halitta guda, kayan aikin software na FCS sun raba sel zuwa ƙungiyoyi daban-daban bisa ga mabanbantan furotin ɗin su.
An nuna wasu sakamakon rigakafin ƙwayoyin cuta a cikin siffa 6
Hoto 6: A: Hoton wakilci na ASCs tare da nau'in halitta mai kama da sanda.OLYMPUS microscope ya ɗauka.Girman asali, (10x).B: Bambance-bambancen Adipogenic na ASCs an tabbatar da shi ta hanyar Ruthenium Red tabo yana nuna wuraren ma'adinai.OLYMPUS microscope ya ɗauka.Girman asali (10x).C: Halin Countstar FL na ASCs.
Taƙaice:
Countstar FL na iya sa ido kan maida hankali, iyawa, da halayen phenotype (da canje-canjen su) yayin samarwa da bambance-bambancen waɗannan sel masu tushe.FCS yana ba da aikin don duba kowane tantanin halitta, inganta bayanai ta hoton.Hakanan mai amfani zai iya samun kwarin gwiwa don aiwatar da gwaje-gwaje na gaba dangane da sakamakon Countstar Rigel.Countstar Rigel yana ba da hanya mai sauri, ƙwaƙƙwalwa, kuma abin dogaro don ingantaccen sarrafa ƙwayoyin kara.
