ʻO nā pūnaewele mesenchymal kahi ʻāpana o nā pūnaewele pluripotent i hiki ke hoʻokaʻawale ʻia mai ka mesoderm.Me kā lākou hoʻololi hou ʻana a me nā hiʻohiʻona ʻokoʻa ʻokoʻa, loaʻa iā lākou ka mana kiʻekiʻe no nā ʻano lapaʻau like ʻole.Loaʻa i nā cell stem Mesenchymal kahi phenotype immune kūʻokoʻa a me ka hiki ke hoʻoponopono pale.No laila, ua hoʻohana nui ʻia nā pūnaewele mesenchymal i ka hoʻololi ʻana i nā cell stem, ka ʻenekinia kiko a me ka hoʻololi ʻana o ke kino.A ma waho aʻe o kēia mau noi, ua hoʻohana ʻia lākou ma ke ʻano he mea hana kūpono i ka ʻenekinia kiko e like me nā cell seeder i loko o kahi ʻano o nā hoʻokolohua noiʻi kumu a me nā lāʻau lapaʻau.
Hiki i ka Countstar Rigel ke nānā i ka manaʻo, viability, apoptosis analysis a me nā hiʻohiʻona phenotype (a me kā lākou mau hoʻololi) i ka wā o ka hana ʻana a me ka hoʻokaʻawale ʻana o kēia mau pūlima stem.Loaʻa i ka Countstar Rigel ka pōmaikaʻi i ka loaʻa ʻana o ka ʻike morphological hou, i hāʻawi ʻia e ke kula mālamalama mau a me nā hoʻopaʻa kiʻi fluorescence i ka wā o ke kaʻina holoʻokoʻa o ka nānā ʻana i ka maikaʻi o ke kelepona.Hāʻawi ka Countstar Rigel i kahi ala wikiwiki, paʻakikī a hilinaʻi hoʻi no ka mālama ʻana i ka maikaʻi o nā pūnaewele stem.
Ka nānā ʻana i ka Viability o nā MSC i ka lāʻau lapaʻau Regenerative
Kiʻi 1 Ka nānā ʻana i ka hiki ʻana a me ka helu cell o nā mesenchymal stem cell (MSCs) no ka hoʻohana ʻana i nā lāʻau lapaʻau.
ʻO Stem Cell kekahi o nā lāʻau lapaʻau maikaʻi loa i nā lāʻau lapaʻau regenerative cell.Mai ka hōʻiliʻili ʻana o MSC a hiki i ka mālama ʻana, he mea nui e hoʻomau i ka hiki ke ola i ka cell stem cell i nā wā āpau o ka hoʻokumu ʻana i nā cell stem (Figure 1).Mālama ka counter cell stem o Countstar i ka hiki ke ola a me ka noʻonoʻo ʻana e pāʻani i ke kuleana koʻikoʻi i ka hoʻokele maikaʻi.
Ka nānā ʻana i nā hoʻololi Morphological MSC ma hope o ka lawe ʻana
Ua hoʻoholo pū ʻia ke anawaena a me ka hōʻuluʻulu ʻana e Countstar Rigel.Ua hoʻololi nui ʻia ke anawaena o nā AdMSC ma hope o ka lawe ʻana i ka wā i hoʻohālikelike ʻia me mua o ka lawe ʻana.ʻO 19µm ka anawaena o mua o ka lawe ʻana, akā ua hoʻonui ʻia i 21µm ma hope o ka lawe ʻana.ʻO ka hōʻuluʻulu o mua o ka lawe ʻana he 20%, akā ua hoʻonui ʻia i 25% ma hope o ka lawe ʻana.Mai nā kiʻi i kiʻi ʻia e Countstar Rigel, ua hoʻololi nui ʻia ka phenotype o AdMSC ma hope o ka lawe ʻana.Ua hōʻike ʻia nā hopena ma ke Kiʻi 3.
Ka ʻike ʻana o nā AdMSC ma Cell Phenotype
I kēia manawa ua helu ʻia nā kaʻina hana hōʻike hōʻike maʻamau no ka hōʻoia maikaʻi o nā MSC i nānā ʻia ma kahi ʻōlelo a ka International Society for Cellular Therapy (ISCT), i wehewehe ʻia ma 2006.
ʻIke wikiwiki o Apoptosis i nā MSC me FITC Conjugated Annexin-V a me 7-ADD Introduction
Hiki ke ʻike ʻia ka Cell Apoptosis me FITC conjugated annexin-V a me 7-ADD.Loaʻa wale ʻia ka PS ma ka lau intracellular o ka membrane plasma i nā sela olakino, akā i ka wā o ka apoptosis mua, nalowale ka asymmetry membrane a unuhi ʻo PS i ka lau waho.
Kiʻi 6 ʻIke ʻia o Apoptosis ma MSC e Countstar Rigel
A. Ka nānā ʻana i ke kiʻi fluorescence o ka ʻike ʻana i ka Apoptosis ma nā MSC
B. E hoʻopuehu i nā ʻāpana o Apoptosis ma nā MSC e FCS express
C. Ka pākēneka o ka heluna pūnaewele ma muli o % maʻamau, % apoptotic, a me % necrotic/hope loa-pae apoptotic cell.
