ʻO Trypan Blue Cell Helu
Ka nānā ʻana a me ka nānā ʻana i ka moʻomeheu cell me nā ʻōnaehana hou.He mea koʻikoʻi ka nānā ʻana i ka hilinaʻi a me ka maikaʻi no ka hoʻomaikaʻi ʻana i ka hua a me ka maikaʻi o ka huahana no ka mea hiki ke hoʻololi liʻiliʻi i nā ʻāpana bioprocess i ka hana o kāu moʻomeheu cell.ʻO ka helu kelepona a me ka viability ka mea koʻikoʻi loa, hāʻawi ʻo Countstar® Altair i kahi akamai loa a hoʻokō pono i ka hopena cGMP no kēia.
I ka wā o ka bioprocess, hoʻohana pinepine ʻia ʻo GFP e hui pū me ka protein recombinant ma ke ʻano he hōʻailona.E hoʻoholo i ka GFP fluorescent hiki ke hōʻike i ka hōʻike protein target.Hāʻawi ʻo Countstar Rigel i kahi hōʻike wikiwiki a maʻalahi no ka hoʻāʻo ʻana i ka transfection GFP a me ka hiki.Ua hoʻopaʻa ʻia nā kelepona me ka Propidium iodide (PI) a me Hoechst 33342 e wehewehe i ka heluna kanaka make a me ka heluna o nā cell cell.Hāʻawi ʻo Countstar Rigel i kahi ala wikiwiki a nui no ka loiloi ʻana i ka maikaʻi o ka hōʻike GFP a me ka hiki i ka manawa like.
Hoʻohana mākou i nā kuki e hoʻonui i kou ʻike i ka wā e kipa aku ai i kā mākou mau pūnaewele: hōʻike mai nā kuki hana iā mākou pehea ʻoe e hoʻohana ai i kēia pūnaewele, nā kuki hana e hoʻomanaʻo i kāu mau makemake a me nā kuki huli ʻana e kōkua iā mākou e kaʻana like i nā ʻike pili iā ʻoe.