I nā manawa o COVID-19 ka nānā ʻana o nā cell mononuclear blood peripheral (PBMCs) a me kā lākou CD-marker patterns he mea nui e hāʻawi i ka ʻikepili koʻikoʻi e hoʻomaopopo maikaʻi i ka piʻi ʻana o kahi maʻi e SARS-CoV-2 i nā kānaka.
ʻO ka maʻamau, he manawa hoʻopau ka nānā ʻana o ka PBMC o nā laʻana koko holoʻokoʻa
kaʻina hana.Hoʻopōkole nui ʻia ka Countstar Rigel i kēia manawa kālele ma o ka hoʻohana ʻana i ke ʻano hoʻopaʻa ʻana AO/PI.Hoʻemi ka polokalamu o ka mea hana i ka helu ʻana i ka hewa a me nā ʻanuʻu i ka nānā hou ʻana (diameter / aggregation rate).
Hāʻawi ka Countstar Rigel i nā hualoaʻa pololei a me ka hoʻohālikelike ʻana me nā kiʻi kiʻekiʻe-kiʻekiʻe o nā cell CD4+ ʻoi aku ka wikiwiki ma mua o ke ala cytometry kahe kuʻuna.Ma waho aʻe o ia mea, ua hōʻike mua nā mea loiloi ʻo Countstar Rigel i ko lākou pololei a me ka hana hou ʻana i nā kaʻina hana cGMP i hoʻoponopono ʻia no nā kano a me nā mea lāʻau lapaʻau ikaika (API) ma ka honua holoʻokoʻa.
E noi i kāu hoa kūʻai kūloko a i ʻole e hoʻokaʻaʻike pololei iā mākou e hoʻonohonoho i kahi demo a i ʻole loiloi o nā hiʻohiʻona Countstar Rigel.Ua mākaukau kā mākou mau loea noi e kōkua iā ʻoe i ka hoʻomaka ʻana a me ke aʻo ʻana.
Fig. 1
ʻO ka ʻāpana o kahi kiʻi ʻālohilohi, i loaʻa mai kahi laʻana PBMC koko holoʻokoʻa e kahi Countstar Rigel S3, loaʻa ka nui o nā ʻōpala, platelets a me nā mea ʻē aʻe i wehewehe ʻole ʻia.
Kiʻi 2
Kiʻi i uhi ʻia, ʻāpana like, nā pūnaewele i hoʻopaʻa ʻia e AO/PI, Kanal 1 (Ex/Em 480nm / 535/40nm) Kanal 2 (Ex/Em: 525nm / 580/25nm : ʻulaʻula: cell dead, Green: cell viable, ʻAlani: mea hōʻailona ʻole, mea kikoʻī ʻole
Kiʻi 3
Hoʻohālikelike ʻia ka ʻikepili cytometry i nā hopena Countstar Rigel, e helu ana i ka hōʻailona CD3-FITC a me CD4-PE o nā cell immune i hoʻoulu ʻia e IL-6
