ʻO ke ʻano kuʻuna o ka helu kelepona ma ka helu lima ma ka hemocytometer.E like me mākou a pau, helu manual me ka hemocytomter i komo i nā ʻanuʻu hewa he nui.ʻO ka pololei o ka hopena e hilinaʻi nui ʻia ma ka ʻike a me ke akamai o nā mea hana.He maʻalahi a maʻalahi ka hoʻohana ʻana i nā helu helu kelepona ʻo Countstar, i hoʻolālā ʻia e hoʻopau i nā hewa i hana ʻia e ke kumu kanaka i ka helu manual a hāʻawi i ka hopena helu helu kelepona kiʻekiʻe a pololei.
Countstar Automated Cell Counters Protocol
1. Hoʻohui i ka hoʻokuʻu ʻana o ke kelepona ma 1: 1 me 0.2% trypan uliuli
2. Inject 20 µL hāpana ma Countstar keena paheʻe.
3. E hoʻouka i ke keʻena helu paheʻe i loko o ka Counstar a nānā
Hoʻohālikelike maʻalahi ʻo Countstar me kahi hemocytometer
Kiʻi A. CHO moʻo dilution helu hopena.Hōʻike nā hualoaʻa Countstar i ka hopena paʻa.Kiʻi B. ʻO ka hoʻoponopono ʻana o ka Countstar a me ka hopena hemocytometer (CHO series dilution).
