ʻO ka loiloi immuno-phenotyping he hoʻokolohua maʻamau i hana ʻia ma nā kahua noiʻi pili pili i ke kelepona no ka ʻike ʻana i nā maʻi like ʻole (maʻi autoimmune, maʻi immunodeficiency, maʻi tumora, hemostasis, maʻi maʻi, a me nā mea hou aku) a me nā maʻi maʻi.Hoʻohana ʻia ia e hoʻāʻo i ka maikaʻi o ke kelepona i nā noiʻi maʻi cell like ʻole.ʻO Flow cytometry a me fluorescence microscope nā ʻano loiloi maʻamau i nā keʻena noiʻi maʻi cell i hoʻohana ʻia no ka immuno-phenotyping.Akā, hiki i kēia mau ʻano loiloi ke hāʻawi i nā kiʻi a i ʻole nā pūnaewele ʻikepili, ʻaʻole hiki ke hoʻokō i nā koi ʻae koʻikoʻi o nā mana hoʻoponopono.
M Dominici el, Cytotherapy (2006) Vol.8, Helu 4, 315-317
Ka ʻike ʻana o ka Immuno-phenotype o AdMSC
Ua hoʻoholo ʻia ka immunophenotype o AdMSC e Countstar FL, ua hoʻokomo ʻia nā AdMSC me nā antibody like ʻole (CD29, CD34, CD45, CD56, CD73, CD105, a me HLADR).Ua hana ʻia kahi kaʻina hana noi kala-hōʻailona ma ka hoʻonohonoho ʻana i ke kahawai ʻōmaʻomaʻo i ke kiʻi PE fluorescence, a me kahi māla mālamalama.Ua hoʻohana ʻia ka māhele kiʻi kiʻi mālamalama ma ke ʻano he mask e hōʻike i ka hōʻailona PE fluorescence.Ua hōʻike ʻia nā hopena o CD105 (Figure 1).
Kiʻi 1 Identification of Immuno-phenotype of AdMSCs.A. Kiʻi Māmā a me Fluorescence o AdMSC;B. CD Marker Detection o AdMSCs na Countstar FL
Ka mana maikaʻi o nā MSC - hōʻoia i nā hopena no kēlā me kēia kelepona
Kiʻi 2 A: Ua hōʻike ʻia nā hualoaʻa Countstar FL ma FCS express 5plus, e loaʻa ana i ka pākēneka maikaʻi o CD105, a me ka nānā ʻana i nā cell single table.B: Hoʻoponopono ʻia ka puka ʻana ma ka ʻaoʻao ʻākau, hōʻike nā kiʻi o ka pākaukau kelepona hoʻokahi i kēlā mau cell me ka hōʻike kiʻekiʻe o CD105.C: Hoʻoponopono ʻia ka puka ma ka ʻaoʻao hema, hōʻike nā kiʻi o ka papa ʻaina hoʻokahi i kēlā mau cell me ka haʻahaʻa o CD105.
Nā Hoʻololi Phenotypical i ka wā o ka lawe ʻana
Kiʻi 3. A: Ka helu helu o ka pākēneka maikaʻi o CD105 i nā laʻana like ʻole e ka polokalamu FCS express 5 plus.B: Hāʻawi nā kiʻi kiʻekiʻe i ka ʻike morphological hou.C: Nā hualoaʻa i hōʻoia ʻia e nā liʻiliʻi liʻiliʻi o kēlā me kēia kelepona hoʻokahi, ua hoʻokaʻawale nā mea lako polokalamu FCS i nā kelepona i ʻokoʻa
