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ʻO ka GFP Transfection Efficiency Assay me ka hoʻohana ʻana i ka 293 a me ka Propidium Iodide

Hoʻolauna

ʻO ka protein fluorescent ʻōmaʻomaʻo (GFP) he pūmua i haku ʻia me 238 mau koena amino acid (26.9 kDa) e hōʻike ana i ka fluorescence ʻōmaʻomaʻo ʻōmaʻomaʻo ke ʻike ʻia i ka mālamalama i ka polū a i ka laulā ultraviolet.I loko o ke kelepona a me ka biology molekula, hoʻohana pinepine ʻia ka gene GFP ma ke ʻano he mea hoʻolaha o ka ʻōlelo.Ma nā ʻano i hoʻololi ʻia, ua hoʻohana ʻia e hana i nā biosensors, a ua hana ʻia nā holoholona he nui e hōʻike ana i ka GFP ma ke ʻano he hōʻoia-o-manaʻo e hiki ke hōʻike ʻia kahi gene i loko o ke kino i hāʻawi ʻia, a i ʻole i loko o nā ʻāpana a i ʻole nā ​​​​pūnaewele a hoihoi paha.Hiki ke hoʻokomo ʻia ka GFP i loko o nā holoholona a i ʻole nā ​​​​mea ʻē aʻe ma o nā ʻenehana transgenic a mālama ʻia i kā lākou genome a me kā lākou mau keiki.

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