ʻO ka helu AOPI Dual-fluoresces ka ʻano assay i hoʻohana ʻia no ka ʻike ʻana i ka neʻe ʻana o ka cell a me ka hiki.ʻO ka hoʻonā ʻana he hui pū ʻana o ka ʻalani acridine (ʻo ka'ōmaʻomaʻo-fluorescent nucleic acid stain) a me propidium iodide (ka ʻulaʻula nucleic acid stain).ʻO ka propidium iodide (PI) he mea hoʻoheheʻe hoʻokaʻawale membrane e komo wale i nā pūnaewele me nā membrane i hoʻopaʻa ʻia, ʻoiai hiki i ka ʻalani acridine ke komo i nā cell āpau i kahi heluna.Ke loaʻa nā wai ʻelua i loko o ka nucleus, ʻo propidium iodide ka mea e hoʻemi ai i ka fluorescence ʻalani acridine ma o ka hoʻololi ʻana i ka ikehu resonance (FRET).ʻO ka hopena, ʻo nā pūnaewele nucleated me nā membrane paʻa i hoʻopaʻa ʻia i ka ʻōmaʻomaʻo ʻōmaʻomaʻo a helu ʻia he ola, ʻoiai nā cell nucleated me nā membrane i hoʻopaʻapaʻa wale ʻia ka ʻulaʻula fluorescent a helu ʻia he make ke hoʻohana ʻia ka ʻōnaehana Countstar® FL.ʻAʻole ʻike ʻia nā mea non-nucleated e like me ke koko ʻulaʻula, platelets a me nā ʻōpala a nānā ʻole ʻia e ka polokalamu Countstar® FL.
Ke Kaʻina Hana o ka Stem Cell Therapy
Kiʻi 4 Ka nānā ʻana i ka ola a me ka helu kelepona o nā pūnaewele mesenchymal stem (MSCs) no ka hoʻohana ʻana i nā lāʻau lapaʻau.
E hoʻoholo i ka pono o ka MSC e AO/PI a me Trypan Blue assay
Kiʻi 2. A. Kiʻi o MSC i hoʻopaʻa ʻia e AO/PI a me Trypan Blue;2. Hoʻohālikelike o AO/PI a me Trypan polū hopena ma mua a ma hope o ka lawe ʻana.
Hoʻololi ka helu helu hoʻololi o ka cell, ʻaʻole maopopo loa ka ʻili ʻana o Trypan Blue, he paʻakikī ke hoʻoholo i ke ola ma hope o ka lawe ʻana.ʻOiai ʻo ka fluorescence ʻelua ʻulaʻula e hiki ai ke hoʻopaʻa ʻia i nā cell nucleated ola a me nā mea make, e hoʻopuka ana i nā hopena kūpono kūpono ʻoiai ke alo o nā ʻōpala, platelets, a me nā ʻulaʻula koko.
