Kūkākūkā hoʻokolohua
Ua helu ʻia ka % cytotoxicity e ka hoohalike ma lalo nei.
Cytotoxicity % = (Nā helu ola o ka hoʻomalu - He helu ola o ka mālama ʻia) / He helu ola o ka mana × 100
Ma ka hoʻopaʻa inoa ʻana i nā cell tumor target me ka non-toxic, non-radioactive calcein AM a i ʻole transfect me GFP, hiki iā mākou ke nānā i ka pepehi ʻia ʻana o nā pūnana maʻi e nā cell CAR-T.ʻOiai e hoʻopaʻa inoa ʻia nā cell cancer target e ka calcein ʻōmaʻomaʻo AM a i ʻole GFP, ʻaʻole hiki i nā mea make ke mālama i ka wai ʻōmaʻomaʻo.Hoʻohana ʻia ʻo Hoechst 33342 no ka hoʻomaʻemaʻe ʻana i nā cell āpau (ʻo nā pūnae T a me nā pūnaewele tumo), ʻo ia hoʻi, hiki ke hoʻopaʻa ʻia nā cell tumor target me ka calcein AM i hoʻopaʻa ʻia me ka membrane, hoʻohana ʻia ʻo PI no ka hoʻopaʻa ʻana i nā pūnana make (ʻelua mau pūnaewele T a me nā pūnaewele tumo).Hiki i kēia hoʻolālā kala ke hoʻokaʻawale i nā cell like ʻole.
E: T Ratio pili i ka Cytotoxicity o K562
ʻO ka laʻana ʻo Hoechst 33342, CFSE, PI fluorescent kiʻi ʻo ia nā kiʻi K562 target ma t = 3 hola.
Ua hōʻike ʻia nā kiʻi fluorescent i ka piʻi ʻana o Hoechst+CFSE+PI+ Target cell i ka piʻi ʻana o ka ratio E: T
