Kev kho ntawm tes yog qhov kev cia siab tshiab uas yuav ua rau yav tom ntej ntawm biomedicine, tab sis kev siv tib neeg lub hlwb hauv cov tshuaj tsis yog lub tswv yim tshiab.Hauv ob peb lub xyoo dhau los, kev kho ntawm tes tau ua tiav zoo, thiab kev kho ntawm tes nws tus kheej tsis yog ib qho yooj yim sau ntawm cov hlwb thiab rov qab los.Cells tam sim no feem ntau yuav tsum tau bioengineered, xws li CAR-T cell therapy.Peb lub hom phiaj yuav muab koj cov qauv, GMP qib cov cuab yeej rau kev tswj hwm ntawm tes.Cov khoom Countstar tau txais los ntawm ntau lub tuam txhab ua cov kev kho ntawm tes, peb tuaj yeem pab peb cov neeg siv khoom tsim kom muaj kev ruaj khov, txhim khu kev qha cell concentration, kev saib xyuas kev ua haujlwm.
Kev sib tw hauv Cell suav thiab kev vam meej
Thaum lub sij hawm tag nrho cov kauj ruam ntawm kev kho mob CAR-T cell manufacturing, lub viability thiab cell suav yuav tsum tau txiav txim precisely.
Freshly cais cov thawj cov hlwb los yog cov kab ke kab lis kev cai yuav muaj cov impurities, ntau hom cell los yog cuam tshuam cov khoom xws li cov khib nyiab ntawm tes uas yuav ua rau nws tsis tuaj yeem txheeb xyuas cov hlwb ntawm kev txaus siab.
Dual Fluorescence viability suav los ntawm Countstar Rigel S2
Acridine txiv kab ntxwv (AO) thiab Propidium iodide (PI) yog nuclear nucleic acid khi dyes.AO tuaj yeem nkag mus rau ob lub hlwb tuag thiab nyob thiab stains cov hlwb nucleated los tsim cov fluorescence ntsuab.PI tuaj yeem stain cov cell nucleated tuag nrog cov kab mob sib kis thiab tsim cov fluorescence liab.Kev tshuaj xyuas tsis suav nrog cov khoom tawg ntawm tes, cov khib nyiab thiab cov khoom pov thawj thiab cov xwm txheej tsis zoo xws li platelets, muab cov txiaj ntsig tau zoo.Hauv kev xaus, Countstar S2 system tuaj yeem siv rau txhua kauj ruam ntawm cov txheej txheem tsim khoom ntawm tes.
A: AO / PI txoj kev tuaj yeem paub qhov txawv ntawm lub cev nyob thiab tuag ntawm cov hlwb, thiab tseem tuaj yeem tshem tawm qhov cuam tshuam.Los ntawm kev sim cov qauv diluting, dual-fluorescence txoj kev qhia tau tias ruaj khov.
Kev txiav txim siab ntawm T / NK Cell Mediated Cytotoxicity
Los ntawm daim ntawv lo rau lub hom phiaj qog hlwb nrog cov tsis muaj tshuaj lom, tsis muaj hluav taws xob calcein AM lossis kis nrog GFP, peb tuaj yeem saib xyuas kev tua cov qog hlwb los ntawm CAR-T hlwb.Thaum cov kab mob qog noj ntshav nyob hauv lub hom phiaj yuav raug sau los ntawm ntsuab calcein AM lossis GFP, cov hlwb tuag tsis tuaj yeem khaws cov xim ntsuab.Hoechst 33342 yog siv rau stain tag nrho cov hlwb (ob leeg T hlwb thiab qog hlwb), lwm yam, lub hom phiaj qog hlwb tuaj yeem stained nrog membrane khi calcein AM, PI yog siv rau stain lub hlwb tuag (ob leeg T hlwb thiab qog hlwb).Lub tswv yim staining no tso cai rau kev ntxub ntxaug ntawm cov hlwb sib txawv.
Kev suav Cell suav nrog thiab Kev Tswj Xyuas Cov Ntaub Ntawv Hauv Ntiaj Teb
Ib qho teeb meem tshwm sim hauv kev suav cov xov tooj ntawm tes yog cov ntaub ntawv sib txawv ntawm cov neeg siv, chaw ua haujlwm thiab chaw.Txhua tus Countstar analyzer suav tib yam hauv qhov chaw sib txawv lossis chaw tsim khoom.Qhov no yog vim hais tias nyob rau hauv tus txheej txheem ntawm kev tswj kom zoo, txhua qhov ntsuas yuav tsum tau calibrated rau tus qauv ntsuas.
Central data bank tso cai rau tus neeg siv khaws tag nrho cov ntaub ntawv, xws li ntsuas ntsuas ntsuas, daim ntawv tshaj tawm xov tooj ntawm tes thiab tus lej ntsuas e-kos npe, nyab xeeb thiab ruaj khov.
Tsheb T Cell Therapy: Kev Cia Siab Tshiab rau Kev Kho Mob Cancer
CAR-T cell therapy yog undoubtedly yog ib tug tshiab kev cia siab rau ua lub neej yav tom ntej ntawm biomedicine rau mob qog noj ntshav.Thaum lub sij hawm tag nrho cov kauj ruam ntawm kev kho mob CAR-T cell manufacturing, lub viability thiab cell suav yuav tsum tau txiav txim precisely.
Lub Countstar Rigel tau txais kev lees paub los ntawm ntau lub tuam txhab ua CAR-T cell therapy, peb tuaj yeem pab peb cov neeg siv khoom tsim kom muaj kev ruaj khov, txhim khu kev qha ntawm tes, kev saib xyuas kev ruaj ntseg.
