Immuno-phenotyping tsom xam yog ib qho kev sim ua nyob rau hauv kev tshawb fawb ntawm tes los kuaj xyuas ntau yam kab mob (autoimmune kab mob, kab mob tiv thaiv kab mob, kab mob qog, hemostasis, kab mob tsis haum, thiab ntau ntxiv) thiab kab mob pathology.Nws kuj yog siv los ntsuas cov cell zoo hauv ntau yam kev tshawb fawb ntawm cov kab mob ntawm tes.Flow cytometry thiab fluorescence microscope yog cov kev soj ntsuam niaj hnub hauv cov kab mob ntawm tes tshawb fawb lub koom haum siv rau kev tiv thaiv kab mob-phenotyping.Tab sis cov kev ntsuam xyuas no tuaj yeem muab cov duab lossis cov ntaub ntawv series, tsuas yog, uas yuav tsis ua raws li kev pom zoo nruj ntawm cov cai tswj hwm.
M Dominici el, Cytotherapy (2006) Vol.8, nr 4, 315-317
Kev txheeb xyuas ntawm Immuno-phenotype ntawm AdMSCs
Lub immunophenotype ntawm AdMSCs tau txiav txim los ntawm Countstar FL, AdMSCs tau incubated nrog txawv antibody raws li (CD29, CD34, CD45, CD56, CD73, CD105, thiab HLADR).Cov txheej txheem kev teeb tsa-xim xim tau tsim los ntawm kev teeb tsa Green channel rau cov duab PE fluorescence, ntxiv rau qhov kaj.Cov duab ci ntsa iab siv segmentation tau siv los ua daim npog ntsej muag rau cov qauv PE fluorescence teeb liab.Cov txiaj ntsig ntawm CD105 tau pom (Daim duab 1).
Daim duab 1 Kev txheeb xyuas ntawm Immuno-phenotype ntawm AdMSCs.A. Bright Field thiab Fluorescence Duab ntawm AdMSCs;B. CD Marker Detection of AdMSCs los ntawm Countstar FL
Kev tswj hwm zoo ntawm MSCs - ua pov thawj cov txiaj ntsig rau txhua lub xov tooj ntawm tes
Daim duab 2 A: Cov txiaj ntsig Countstar FL tau tshwm sim hauv FCS nthuav qhia 5plus, suav qhov feem pua zoo ntawm CD105, thiab saib cov lus qhia ib leeg.B: Kho qhov rooj mus rau sab xis, cov duab ntawm ib lub xov tooj ntawm tes qhia cov hlwb nrog kev qhia siab ntawm CD105.C: Kho qhov rooj rau sab laug, cov duab ntawm ib lub hlwb lub rooj qhia cov hlwb uas tsis tshua muaj kev qhia ntawm CD105.
Phenotypical Hloov thaum thauj
Daim duab 3. A: Kev txheeb xyuas qhov feem pua zoo ntawm CD105 hauv cov qauv sib txawv los ntawm FCS nthuav qhia 5 ntxiv rau software.B: Cov duab zoo siab muab cov ntaub ntawv morphological ntxiv.C: Cov txiaj ntsig tau lees paub los ntawm cov duab me me ntawm txhua lub xov tooj ntawm tes, FCS software cov cuab yeej faib cov hlwb rau hauv qhov sib txawv
