A na-eji mkpụrụ ndụ mammalian eme ihe na Biopharmaceuticals, dị ka ọgwụ mgbochi, ọgwụ mgbochi, peptides na metabolites nke abụọ na-emepụta site na bioprocessing na mkpụrụ ndụ anụ mammalian.N'ime usoro niile site na mgbochi R&D ruo mmepụta, enwere ọtụtụ usoro achọrọ iji mee nyocha dabere na cell iji nyochaa usoro ma ọ bụ njikwa mma.Dị ka mkpokọta mkpokọta cell na ịdị ndụ ga-akọwapụta ọnọdụ omenala sel.Yana mbufe cell, mgbochi mgbochi na-ekpebi n'ogo cell.Ngwa Countstar bụ cytometry dabere na onyonyo, nwere ike inye aka nyochaa site na R&D ruo na usoro mmepụta ma hụ na mmụgharị na nkwụsi ike.
Ọnụọgụ mkpụrụ ndụ yana ịdị ndụ site n'ụkpụrụ nchacha anụnụ anụnụ Trypan
Nleba anya na nyochaa omenala cell na ihe ngwọta ọgbara ọhụrụ.Nlebanya ntụkwasị obi na nke ọma dị oke mkpa maka ịkwalite mkpụrụ na ịdị mma ngwaahịa ebe ọ bụ na ọbụlagodi obere mgbanwe na paramita bioprocess nwere ike imetụta arụmọrụ nke omenala cell gị.Ọnụọgụ cell na ịdị ndụ bụ paramita kachasị mkpa, Countstar Altair na-enye amamihe dị oke egwu ma na-agbaso usoro cGMP maka ndị a.
Emebere Countstar Altair dabere n'ụkpụrụ mwepu nke Trypan Blue, na-ejikọta bench anya onyonyo anya “dozi anya” dị elu, teknụzụ njirimara cell kachasị elu na algọridim ngwanrọ.Kwado ịnweta ozi gbasara mkpokọta cell, ịdị ndụ, ọnụ ọgụgụ nchịkọta, okirikiri, na nkesa dayameta site n'otu ọsọ.
Viability na Mkpebi Mbufe GFP na sel
N'oge bioprocess, a na-ejikarị GFP ejikọta ya na protein recombinant dị ka ihe ngosi.Kpebisie ike na GFP fluorescent nwere ike igosipụta nkwupụta protein ebumnuche.Countstar Rigel na-enye nyocha ngwa ngwa ma dị mfe maka ịnwale mbufe GFP yana ike.Ejiri Propidium iodide (PI) na Hoechst 33342 nwere ntụpọ mkpụrụ ndụ iji kọwaa ọnụ ọgụgụ sel nwụrụ anwụ na mkpokọta ọnụ ọgụgụ cell.Countstar Rigel na-enye usoro ọsọ ọsọ, ọnụọgụ maka ịlele arụmọrụ okwu GFP na ịdị adị n'otu oge.
Selụ dị site na iji Hoechst 33342 (acha anụnụ anụnụ) na pasent nke GFP na-egosipụta mkpụrụ ndụ (akwụkwọ ndụ akwụkwọ ndụ) nwere ike ikpebi ngwa ngwa.A na-eji propidium iodide (PI; acha ọbara ọbara).
Mmekọrịta nke nchọpụta mgbochi mmadụ na Countstar Rigel
Gwakọta ihe mgbochi ahụ n'ime ebe dị iche iche, wee tinye ya na mkpụrụ ndụ.E nwetara nsonaazụ ya site na Countstar Rigel (ma onyonyo yana nsonaazụ ọnụọgụ)
Countstar dị GMP-njikere maka 21 CFR Nkebi 11
Ngwa Countstar na-akwado nke ọma na 21 CFR na Nkebi 11, ọrụ IQ/OQ/PQ na-ahụ maka njikwa ọrụ na-agbanwe agbanwe.Ngwa Countstar dị njikere etinyere ya na GMP na 21 CFR akụkụ 11 ụlọ nyocha.Ụzọ njikwa na nyocha nke onye ọrụ na-enye ohere maka akwụkwọ eji eme ihe zuru oke yana mkpesa PDF ahaziri ahazi.
Akwụkwọ IQ/OQ na akụkụ nkwado
