Ihe nlele nwere sel na nkwusioru bụ ịgwakọta na Trypan blue dye, wee dọba ya na Countstar Chamber Slide nke Countstar Automated Cell Counter nyochara.Dabere na ụkpụrụ ọnụ ọgụgụ cell trypan acha anụnụ anụnụ, ngwa Countstar na-ejikọta teknụzụ nyocha ngwa anya dị elu, teknụzụ njirimara onyonyo na ngwanrọ sọftụwia dị ike ọ bụghị naanị na-enye ntinye mkpụrụ ndụ na ịdị ndụ, kamakwa na-enye ozi nke mkpokọta cell, viability, mkpokọta mkpokọta, okirikiri. , na dayameta nkesa na naanị otu ọsọ.
Analysis Cell agbakọtara
Ọgụgụ 3 Ngụkọta mkpụrụ ndụ agbakọtara.
A. Ihe onyonyo nke ihe nlele cell;
B. Onyonyo nke Sample cell nwere akara njirimara nke sọftụwia Countstar BioTech.(Green Circle: Live cell, Yellow Circle: Dead cell, Red Circle: Aggregated Cell).
C. Histogram gbakọtara
Ụfọdụ mkpụrụ ndụ ndị bụ isi ma ọ bụ sel subculture na-enwekarị ike ịgbakọta mgbe ọdịbendị na-adịghị mma ma ọ bụ mgbari mgbaze nke ukwuu, si otú a na-akpata nnukwu nsogbu n'ịgụ mkpụrụ ndụ.Site na Ọrụ Calibration Aggregation, Countstar nwere ike ịghọta ngụkọ mkpali nke mkpokọta iji hụ na ọnụọgụ mkpụrụ ndụ ziri ezi wee nweta ọnụọgụ mkpokọta na mkpokọta mkpokọta, si otú a na-enye ihe ndabere maka ndị nnwale ikpe ikpe steeti sel.
Nleba anya na-eto eto cell
Onyonyo 4 Usoro eto eto cell.
Usoro uto mkpụrụ ndụ bụ usoro a na-ahụkarị maka ịlele uto zuru oke nke ọnụọgụ cell, ihe dị mkpa na-egosi itinye uche cell na otu n'ime ihe ndị bụ isi maka ọdịbendị nke ihe ndị dị ndụ nke mkpụrụ ndụ.Iji kọwaa n'ụzọ ziri ezi mgbanwe dị ike na ọnụ ọgụgụ nke mkpụrụ ndụ n'ime usoro dum, enwere ike kewaa usoro uto na-ahụkarị n'ime akụkụ anọ: oge incubation na-eto ngwa ngwa;Oge uto na-enweghị atụ nwere nnukwu mkpọda, oge ala dị larịị na oge ọdịda.Enwere ike nweta usoro uto cell site n'ichepụta ọnụ ọgụgụ nke mkpụrụ ndụ dị ndụ (10'000 / ml) megide oge omenala (h ma ọ bụ d).
Na-atụ mkpokọta cell na ike
Countstar BioTech weghaara onyonyo 1 dị ka mkpụrụ ndụ (Vero, 3T3, 549, B16, CHO, Hela, SF9, na MDCK) na nkwusioru nke Trypan Blue mebere n'otu n'otu.
Countstar na-adabara mkpụrụ ndụ nwere dayameta n'etiti 5-180um, dị ka cell mammalian, cell ụmụ ahụhụ, na ụfọdụ planktons.
Nleba nha cell
Ọgụgụ 2 Nleba nha cell nke mkpụrụ ndụ CHO tupu na mgbe mbufe plasmid gasịrị.
A. Onyonyo nke nkwusioru sel CHO nwere ntụpọ na-acha anụnụ anụnụ trypan tupu na mgbe mbufe plasmid gasịrị.
B. Ntụnyere histogram nha cell CHO tupu na mgbe mbufe plasmid gasịrị.
Mgbanwe nha cell bụ isi ihe a na-atụkarị ya na nyocha cell.Dị ka ọ na-adịkarị, a ga-atụ ya na nnwale ndị a: nnyefe cell, nyocha ọgwụ na nyocha ọrụ cell.Countstar na-enye data morphology ọnụ ọgụgụ, dị ka nha nke mkpụrụ ndụ, n'ime 20s.
Ọnụ ọgụgụ cell Countstar akpaghị aka nwere ike ịnye data morphological nke mkpụrụ ndụ, gụnyere okirikiri na dayameta histogram.
