Selụ sel mesenchymal bụ akụkụ nke mkpụrụ ndụ stem pluripotent nke enwere ike kewapụ na mesoderm.Site na mmeghari ohuru nke onwe ha na njirimara di iche iche nke uzo, ha nwere ikike di elu maka usoro ogwu di iche iche na ogwu.Selụ sel mesenchymal nwere phenotype nke na-alụso ọrịa ọgụ na ikike ịchịkwa usoro ahụ ji alụso ọrịa ọgụ.Ya mere, a na-eji mkpụrụ ndụ mesenchymal eme ihe n'ọtụtụ ebe na ntụgharị cell cell transplantation, nhazi anụ ahụ na ntụgharị akụkụ ahụ.Na gafere ngwa ndị a, a na-eji ha dị ka ezigbo ngwá ọrụ na injinịa anụ ahụ dị ka mkpụrụ ndụ mkpụrụ osisi na usoro nyocha nyocha nke isi na nke ụlọ ọgwụ.
Countstar Rigel nwere ike nyochaa itinye uche, viability, nyocha apoptosis na njirimara phenotype (na mgbanwe ha) n'oge mmepụta na ọdịiche nke sel ndị a.Countstar Rigel nwekwara uru dị n'inweta ozi morphological ndị ọzọ, nke mpaghara na-egbuke egbuke na-adịgide adịgide na ndekọ onyonyo dabere na fluorescence n'oge usoro nlekota ogo cell.Countstar Rigel na-enye usoro ọsọ ọsọ, ọkaibe na nke a pụrụ ịdabere na ya maka njikwa mma nke sel sel.
Nleba anya ike nke ndị MSC na ọgwụ na-emegharị ahụ
Ọgụgụ 1 Nyochaa viability na ọnụ ọgụgụ cell nke mesenchymal stem cell (MSCs) maka ojiji na usoro ọgwụgwọ cell.
Cell Stem bụ otu n'ime ọgwụgwọ kachasị mma na usoro ọgwụgwọ sel na-emegharị ahụ.Site na owuwe ihe ubi nke MSC ruo na ọgwụgwọ, ọ dị mkpa ịkwado ikike cell stem dị elu n'oge usoro niile nke mmepụta cell stem (Nyocha 1).Countstar's stem cell counter na-enyocha ike ndụ cell na itinye uche ka ọ rụọ ọrụ dị oke mkpa na njikwa mma.
Nleba anya MSC Mgbanwe Mgbanwe Mgbanwe ka ụgbọ njem gachara
Countstar Rigel kpebikwara dayameta na mkpokọta.Agbanwere dayameta nke AdMSC nke ukwuu mgbe ụgbọ njem gachara ma atụnyere tupu ebufee ya.Dayameta nke tupu ebuga bụ 19µm, mana ọ gbagoro na 21µm mgbe ụgbọ njem gachara.Nchịkọta nke tupu ebuga bụ 20%, mana ọ mụbara ruo 25% mgbe ụgbọ njem gasịrị.Site na onyonyo nke Countstar Rigel weghaara, phenotype nke AdMSC gbanwere nke ukwuu ka emechara njem.E gosipụtara nsonaazụ ya na eserese 3.
Nchọpụta AdMSC na Cell Phenotype
Ugbu a usoro ule njirimara ọkọlọtọ kacha nta maka mmesi obi ike nke ndị MSC na-enyocha ka edepụtara na nkwupụta nke International Society for Cellular Therapy (ISCT), kọwapụtara ugbua na 2006.
Nchọpụta Apoptosis ngwa ngwa na MSC nwere FITC Conjugated Annexin-V na 7-ADD Okwu Mmalite.
Enwere ike ịhụ Apoptosis cell site na FITC conjugated annexin-V na 7-ADD.A na-ahụkarị PS naanị na mpempe akwụkwọ intracellular nke akpụkpọ ahụ plasma na mkpụrụ ndụ ahụike, mana n'oge apoptosis n'oge, akpụkpọ ahụ asymmetry na-efunahụ ma PS na-atụgharị gaa na mpempe akwụkwọ mpụga.
Ọgụgụ 6 Nchọpụta Apoptosis na MSC site n'aka Countstar Rigel
A. Nlele anya nke onyonyo fluorescence nke Nchọpụta Apoptosis na MSC
B. Gbasaa nkata Apoptosis na MSC site na FCS express
C. Pasent nke ọnụ ọgụgụ sel dabere na % nkịtị, % apoptotic, na % necrotic/nke dị n'azụ oge sel apoptotic.
