Ụlọ » Maka Bioprocessing

Ihe anyị nwere ike ime

  • Ịgụ mkpụrụ ndụ Trypan Blue
  • Viability na Mbufe GFP
  • Mgbakwunye mgbochi ọrịa
Trypan Blue Cell Counting
Ịgụ mkpụrụ ndụ Trypan Blue

Ịgụ mkpụrụ ndụ Trypan Blue

Nleba anya na nyochaa omenala cell na ihe ngwọta ọgbara ọhụrụ.Nlebanya ntụkwasị obi na nke ọma dị oke mkpa maka ịkwalite mkpụrụ na ịdị mma ngwaahịa ebe ọ bụ na ọbụlagodi obere mgbanwe na paramita bioprocess nwere ike imetụta arụmọrụ nke omenala cell gị.Ọnụọgụ cell na ịdị ndụ bụ paramita kachasị mkpa, Countstar® Altair na-enye amamihe dị oke egwu ma na-agbaso usoro cGMP nke ọma maka ndị a.

Viability and GFP Transfection
Viability na Mbufe GFP

N'oge bioprocess, a na-ejikarị GFP ejikọta ya na protein recombinant dị ka ihe ngosi.Kpebisie ike na GFP fluorescent nwere ike igosipụta nkwupụta protein ebumnuche.Countstar Rigel na-enye nyocha ngwa ngwa ma dị mfe maka ịnwale mbufe GFP yana ike.Ejiri Propidium iodide (PI) na Hoechst 33342 nwere ntụpọ mkpụrụ ndụ iji kọwaa ọnụ ọgụgụ sel nwụrụ anwụ na mkpokọta ọnụ ọgụgụ cell.Countstar Rigel na-enye usoro ọsọ ọsọ, ọnụọgụ maka ịlele arụmọrụ okwu GFP na ịdị adị n'otu oge.

Antibodies Affinity
Mgbakwunye mgbochi ọrịa
Elisa ma ọ bụ Biacore na-atụkarị ọgwụ mgbochi mmekọrịta, ụzọ ndị a na-enwe mmetụta nke ukwuu, mana ha na-achọpụta mgbochi ahụ nwere protein dị ọcha, mana ọ bụghị protein ngbanwe eke.Jiri usoro immunofluorescence cell, onye ọrụ nwere ike ịchọpụta njikọ mgbochi mmadụ na protein conformation eke.Ka ọ dị ugbu a, a na-enyocha cytometry eruba n'ịkọwa njikọ nke mgbochi.Countstar Rigel nwekwara ike ịnye ụzọ dị mfe ma dị mfe iji nyochaa njikọ nke mgbochi.
Countstar Rigel nwere ike weghara onyonyo a na-akpaghị aka wee wepụta oke ọkụ nke nwere ike igosipụta mmekọ nwoke na nwanyị.

Ngwaahịa akwadoro

Countstar Altair

Countstar Altair

Ezubere maka iji kwa ụbọchị na gburugburu cGMP ahaziri
  • Ike njikwa data ezoro ezo
  • Nweta na njikwa onye ọrụ ọkwa anọ
  • E-mbinye aka na faịlụ ndekọ na nnabata zuru oke na FDA 21 CFR Nkebi 11
    • Countstar Rigel S2

    Countstar Rigel S2

    Igwe nyocha nke fluorescence
  • Na-ejikọta fluorescence abụọ gbakwunyere na ọwa na-egbuke egbuke
  • Maka ihe gbasara sel isi, ihe nlele ọbara dị n'akụkụ, CAR-T, NK, na sel MSC
  • Nkwenye maka mmezu nke ule dị mfe
    • Countstar Rigel S3

    Countstar Rigel S3

    Ngwọta zuru ụwa ọnụ maka ịgụta mkpụrụ ndụ, ịkpa oke ndụ ndụ mkpụrụ ndụ, yana nyocha Cytotoxicity nke T/NK Cell gbakọrọ.
  • Na-ejikọta fluorescence atọ na ọwa na-egbuke egbuke
  • Sistemụ onyonyo ekwentị na-akpaghị aka, nke ngwa na-achị
  • Ezubere iji mee ka ọrụ ụlọ nyocha cell dị mfe
    • Countstar BioTech

    Ọnụ ego nke Countstar BioTech

    Onye nyocha gị ziri ezi na ntụkwasị obi na nlekota mmepụta omenala cell
  • Nyochaa nlele ugboro ugboro, ka ngwanro ahụ gbakọọ nkezi nke ụdị dị iche iche, wee tulee data nke nyocha otu
  • ruo sel 2,000 n'ime otu onyonyo Countstar BioTech
  • Nyochaa kpọmkwem otu mkpụrụ ndụ ọbụlagodi n'ime mkpokọta mkpokọta
  • Jiri parampat ndị enyere na-akpaghị aka dị ka dayameta cell, okirikiri cell na mkpokọta mkpokọta
    • Countstar BioMed

    Countstar BioMed

    Onye nyocha maka nleba anya cell cell, nyocha nzaghachi usoro ahụ ji alụso ọrịa ọgụ, nyocha ọrịa kansa, mmepe usoro ọgwụgwọ sel, yana nyocha PBMC.
  • Na-achọpụta ma na-ekewa obere sel anụ mammali na ntụkwasị obi n'ọhịa na-egbuke egbuke (Mwepu Tripan Blue)
  • Algọridim usoro nyocha ihe onyonyo dị elu gụnyere nchịkọta nchịkọta
  • njikwa ohere onye ọrụ cGMP, mbinye aka eletrọnịkị, mkpesa nsonaazụ yana faịlụ ndekọ sistemụ
  • Akụkọ nsonaazụ PDF nwere ike ịhazi ya

    • Ihe ndị metụtara ya

    Nyocha Cytotoxicity T/NK Cell Mediated site na CFSE, Hoechest 33342 na PI

    Protocol nnwale A na-agbakọ cytotoxicity% site na nha anya dị n'okpuru.Cytotoxicity% = (ọnụọgụ njikwa ndụ - Ọnụọgụ nke ọgwụgwọ) / L…

    Ọnwa Iri na Abụọ 30, 2020

    Nzuzo gị dị anyị mkpa.

    Anyị na-eji kuki iji kwalite ahụmịhe gị mgbe ị na-eleta weebụsaịtị anyị: kuki arụmọrụ na-egosi anyị otu esi eji webụsaịtị a, kuki na-arụ ọrụ na-echeta mmasị gị na kuki ezubere iche na-enyere anyị aka ịkekọrịta ọdịnaya dị gị mkpa.

    Nabata

    Banye