Immuno-phenotyping analysis bụ nnwale a na-ahụkarị na ngalaba nyocha metụtara cell iji chọpụta ọrịa dị iche iche (ọrịa autoimmune, ọrịa immunodeficiency, ọrịa tumor, hemostasis, ọrịa nrịanrịa, na ọtụtụ ndị ọzọ) na pathology ọrịa.A na-ejikwa ya iji nwalee ogo cell na nyocha ọrịa sel dị iche iche.cytometry na-efe efe na microscope fluorescence bụ usoro nyocha oge na-aga n'ihu na ụlọ ọrụ nyocha ọrịa mkpụrụ ndụ ejiri maka immuno-phenotyping.Mana ụzọ nyocha ndị a nwere ike ịnye onyonyo ma ọ bụ usoro data, naanị, nke nwere ike ọ gaghị emezu ikike nnabata siri ike nke ndị isi nchịkwa.
Mi Dominici el, Cytotherapy (2006) Vol.8, Nke 4, 315-317
Nchọpụta Immuno-phenotype nke AdMSC
Countstar FL kpebisiri ike na ụdị immunophenotype nke AdMSC, ndị AdMSC bu ihe mgbochi dị iche iche (CD29, CD34, CD45, CD56, CD73, CD105, na HLADR).Emepụtara usoro ngwa mgbaama site na ịtọ ọwa Green ka ọ bụrụ onyonyo PE fluorescence, gbakwunyere ubi na-egbuke egbuke.A na-etinye akụkụ ntụaka foto na-egbuke egbuke dị ka ihe nkpuchi iji gosipụta akara ngosi PE fluorescence.E gosiputara nsonaazụ CD105 (Foto 1).
Ọgụgụ 1 Nchọpụta Immuno-phenotype nke AdMSC.A. Ubi na-egbuke egbuke na Onyonyo Fluorescence nke AdMSC;B. Ihe nrịbama CD nke AdMSC sitere na Countstar FL
Njikwa ogo nke MSC - na-akwado nsonaazụ maka otu sel ọ bụla
Ọnụọgụ 2 A: E gosipụtara nsonaazụ Countstar FL na FCS express 5plus, na-enweta pasentị dị mma nke CD105, yana nyocha otu mkpụrụ ndụ tebụl.B: Gating na-agbanwe n'akụkụ aka nri, ihe oyiyi nke tebụl otu cell na-egosi sel ndị ahụ nwere nkwupụta dị elu nke CD105.C: Agbanwe agbaziri n'akụkụ aka ekpe, onyonyo nke tebụl otu mkpụrụ ndụ na-egosi sel ndị ahụ nwere obere okwu CD105.
Mgbanwe Phenotypical n'oge Ụgbọ njem
Onyonyo 3. A: Ntụle ọnụọgụgụ nke pasent dị mma nke CD105 na nlele dị iche iche site na FCS express 5 plus software.B: Onyonyo dị elu na-enye ozi morphological ọzọ.C: Nsonaazụ enwetara site na mkpịsị mkpịsị mkpịsị aka nke otu cell, ngwa ngwa FCS kewara mkpụrụ ndụ n'ime dị iche iche.
