Kunyumba » Mapulogalamu » Njira Yapawiri ya Fluorescence Kusanthula Magazi ndi Maselo Oyambirira

Njira Yapawiri ya Fluorescence Kusanthula Magazi ndi Maselo Oyambirira

Magazi ndi ma cell omwe angodzipatula okha kapena maselo otukuka amatha kukhala ndi zonyansa, mitundu ingapo ya maselo kapena tinthu tating'onoting'ono tomwe timakhala ngati zinyalala zama cell zomwe zingapangitse kuti zikhale zosatheka kusanthula ma cell a chidwi.Countstar FL yokhala ndi njira ziwiri zowunika za fluorescence imatha kusiyanitsa zidutswa za cell, zinyalala ndi tinthu tating'onoting'ono komanso zochitika zochepa monga mapulateleti, zomwe zimapereka zolondola kwambiri.

 

 

AO/PI Dual Fluorescence Viability Kuwerengera

 

Acridine orange (AO) ndi Propidium iodide (PI) ndi nyukiliya nucleic acid kumanga utoto.Kuwunikaku sikuphatikiza zidutswa za cell, zinyalala ndi tinthu tating'onoting'ono komanso zochitika zochepa monga maselo ofiira a magazi, zomwe zimapereka zotsatira zolondola kwambiri.Pomaliza, dongosolo la Countstar litha kugwiritsidwa ntchito pagawo lililonse la kupanga ma cell.

 

 

Ma WBC mu Magazi Onse

Chithunzi 2 Chithunzi chamagazi athunthu chojambulidwa ndi Countstar Rigel

 

Kusanthula ma WBCs m'magazi athunthu ndi kuyesa kokhazikika mu labu yachipatala kapena nkhokwe ya magazi.Kukhazikika ndi kuthekera kwa ma WBCs ndizomwe zimafunikira pakuwongolera kusungidwa kwa magazi.

The Countstar Rigel yokhala ndi njira ya AO/PI imatha kusiyanitsa bwino lomwe ma cell amoyo ndi akufa.Rigel imathanso kuwerengera WBC molondola ndikupatula kusokoneza kwa maselo ofiira a magazi.

 

 

Kuwerengera ndi Kutheka kwa PBMC

Chithunzi cha 3 Bright Field ndi Fluorescence zithunzi za PBMC Zogwidwa ndi Countstar Rigel

 

Kuwerengera kwa AOPI Dual-fluoresces ndi mtundu woyeserera womwe umagwiritsidwa ntchito pozindikira kuchuluka kwa ma cell ndi kuthekera kwake.Zotsatira zake, ma cell a nucleated okhala ndi nembanemba osawoneka bwino amadetsa zobiriwira za fulorosenti ndipo amawerengedwa kuti ndi amoyo, pomwe ma cell a nucleated okhala ndi nembanemba osokonekera amangopaka utoto wofiira wa fulorosenti ndipo amawerengedwa ngati akufa akamagwiritsa ntchito Countstar Rigel system.Zinthu zopanda ma nucleated monga maselo ofiira a magazi, mapulateleti ndi zinyalala sizimatuluka ndipo zimanyalanyazidwa ndi pulogalamu ya Countstar Rigel.

 

 

 

 

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