Chitsanzo chokhala ndi ma cell oyimitsidwa ndikusakanikirana ndi utoto wa buluu wa Trypan, kenako kukokedwa mu Countstar Chamber Slide yowunikidwa ndi Countstar Automated Cell Counter.Kutengera mfundo yowerengera ma cell a trypan buluu, zida za Countstar zimaphatikiza ukadaulo wapamwamba woyerekeza, ukadaulo wanzeru wozindikira zithunzi komanso ma algorithms amphamvu apulogalamu kuti asamangopereka kuchuluka kwa ma cell komanso kuthekera, komanso kupereka chidziwitso cha kuchuluka kwa ma cell, kuthekera, kuchulukana, kuzungulira. , ndi kugawa m'mimba mwake ndi kuthamanga kumodzi kokha.
Aggregated Cell Analysis
Chithunzi 3 Kuwerengera kwa ma cell ophatikizana.
A. Chithunzi cha Zitsanzo za Maselo;
B. Chithunzi cha Cell Sample yokhala ndi chizindikiritso cha pulogalamu ya Countstar BioTech.(Green Circle: Selo yamoyo, Yellow Circle: Selo yakufa, Red Circle: Aggregated Cell).
C. Aggregated Histogram
Ma cell ena oyambilira kapena ma cell a subculture amatha kuphatikizika ngati chikhalidwe chosauka bwino kapena chimbudzi cham'mimba kwambiri, zomwe zimapangitsa kuti pakhale vuto lalikulu pakuwerengera ma cell.Ndi Aggregation Calibration Function, Countstar imatha kuzindikira kuwerengera kolimbikitsa kwa ma aggregations kuti atsimikizire kuwerengera molondola kwa maselo ndikupeza kuchuluka kwa ma aggregation ndi histogram yophatikiza, motero kumapereka maziko kwa oyesera kuweruza momwe ma cell akhalira.
Kuyang'anira Kukula Kwa Maselo
Chithunzi 4 Cell Grow Curve.
Cell kukula pamapindikira ndi njira wamba kuyeza mtheradi kukula kwa chiwerengero cha maselo, chizindikiro chofunika kudziwa ndende ya maselo ndi chimodzi mwa zofunika magawo a chikhalidwe cha zofunika kwachilengedwenso katundu maselo.Pofuna kufotokozera bwino kusintha kwachangu kwa chiwerengero cha maselo mu ndondomeko yonseyi, mayendedwe a kukula kwake akhoza kugawidwa m'magawo a 4: nthawi ya incubation ndi kukula pang'onopang'ono;gawo la kukula kwakukulu ndi malo otsetsereka, gawo lamapiri ndi nthawi yotsika.Mzere wa kukula kwa selo ukhoza kupezeka pokonza chiwerengero cha maselo amoyo (10'000 / mL) motsutsana ndi nthawi ya chikhalidwe (h kapena d).
Kuyeza kuchuluka kwa ma cell ndi kuthekera kwake
Chithunzi 1 Zithunzi zidajambulidwa ndi Countstar BioTech ngati ma cell (Vero, 3T3, 549, B16, CHO, Hela, SF9, ndi MDCK) pakuyimitsidwa adayimitsidwa ndi Trypan Blue motsatana.
Countstar imagwira ntchito pama cell okhala pakati pa 5-180um, monga ma cell a mammalian, cell cell, ndi planktons.
Kuyeza Kukula Kwa Maselo
Chithunzi cha 2 Kuyeza Kukula kwa Maselo a maselo a C O musanayambe komanso pambuyo pa kupatsirana kwa plasmid.
A. Zithunzi zama cell a CHO kuyimitsidwa ndi buluu wa trypan asanayambe komanso pambuyo pa kufalikira kwa plasmid.
B. Kuyerekeza kwa CHO cell size histogram isanayambe komanso itatha kufalikira kwa plasmid.
Kusintha kwa kukula kwa maselo ndi chinthu chofunikira kwambiri ndipo nthawi zambiri chimayesedwa mu kafukufuku wa maselo.Nthawi zambiri idzayezedwa muzoyesera izi: kutumizirana ma cell, kuyesa mankhwala ndi kuyesa ma cell activation.Countstar imapereka chidziwitso cha morphology, monga kukula kwa maselo, mkati mwa 20s.
Countstar automated cell counter imatha kupereka chidziwitso cha ma cell, kuphatikiza ma circularity ndi diameter histogram.
