Kuchiza kwa ma cell mosakayikira ndi chiyembekezo chatsopano chotsogolera tsogolo la biomedicine, koma kugwiritsa ntchito maselo aumunthu muzamankhwala si lingaliro latsopano.M'zaka makumi angapo zapitazi, chithandizo cha ma cell chapita patsogolo kwambiri, ndipo ma cell therapy sakhalanso gulu losavuta la maselo ndikulowetsedwa mmbuyo.Maselo tsopano amafunikira kuti apangidwe ndi bioengineered, monga CAR-T cell therapy.Tikufuna kukupatsirani zida zokhazikika, za GMP zowongolera ma cell.Chogulitsa cha Countstar chavomerezedwa ndi makampani ambiri omwe akutsogolera chithandizo cha ma cell, titha kuthandiza makasitomala athu kuti apange makina okhazikika, odalirika a cell, makina owunikira.
Kuvuta pa kuchuluka kwa ma cell ndi kuthekera kwake
Pamasitepe onse opanga ma cell a CAR-T, kuthekera ndi kuchuluka kwa maselo kuyenera kutsimikiziridwa bwino.
Maselo omwe angodzipatula okha kapena maselo otukuka amatha kukhala ndi zonyansa, mitundu ingapo ya maselo kapena tinthu tating'onoting'ono tomwe timakhala ngati zinyalala zama cell zomwe zimapangitsa kuti zikhale zosatheka kusanthula ma cell omwe amawakonda.
Kuwerengera kwapawiri kwa Fluorescence kolemba ndi Countstar Rigel S2
Acridine orange (AO) ndi Propidium iodide (PI) ndi nyukiliya nucleic acid kumanga utoto.AO imatha kulowa m'maselo onse akufa komanso amoyo ndikuyika ma cell a nucleated kuti apange fulorosenti yobiriwira.PI imatha kuyipitsa ma cell okhala ndi ma nucleated okhala ndi nembanemba osokonekera ndikupanga fluorescence yofiira.Kuwunikaku sikuphatikiza zidutswa za cell, zinyalala ndi tinthu tating'onoting'ono komanso zochitika zocheperako monga mapulateleti, zomwe zimapereka zotsatira zolondola kwambiri.Pomaliza, dongosolo la Countstar S2 litha kugwiritsidwa ntchito pagawo lililonse la kupanga ma cell.
A: Njira ya AO/PI imatha kusiyanitsa molondola momwe maselo amoyo ndi akufa, komanso amasiya kusokoneza.Poyesa zitsanzo za diluting, njira yapawiri-fluorescence imawonetsa zotsatira zokhazikika.
Kutsimikiza kwa T / NK Cell Mediated Cytotoxicity
Polemba ma cell a chotupa omwe ali ndi calcein AM yopanda poizoni, yopanda ma radio kapena transfect ndi GFP, titha kuyang'anira kuphedwa kwa ma cell a chotupacho ndi ma cell a CAR-T.Ngakhale maselo a khansa omwe akufuna kukhala ndi moyo adzalembedwa ndi green calcein AM kapena GFP, maselo akufa sangathe kusunga utoto wobiriwira.Hoechst 33342 imagwiritsidwa ntchito pakudetsa ma cell onse (ma cell a T ndi cell chotupa), m'malo mwake, ma cell chandamale amatha kuipitsidwa ndi nembanemba womangidwa calcein AM, PI imagwiritsidwa ntchito pakudetsa ma cell akufa (ma cell a T ndi ma cell chotupa).Njira yodetsa iyi imalola kusankhana kwa ma cell osiyanasiyana.
Kuwerengera Ma Cell Mogwirizana ndi Kusamalira Data Kwapadziko Lonse
Vuto lodziwika bwino pakuwerengera ma cell ndi kusiyana kwa data pakati pa ogwiritsa ntchito, madipatimenti ndi masamba.Onse Countstar analyzer amawerengera chimodzimodzi m'malo osiyanasiyana kapena malo opangira.Izi zili choncho chifukwa pakuwongolera khalidwe, chida chilichonse chiyenera kusinthidwa kukhala chida chokhazikika.
Bank data yapakati imalola wogwiritsa ntchito kusunga zidziwitso zonse, monga lipoti loyesa zida, lipoti lachitsanzo cha cell ndi siginecha ya tester, yotetezeka komanso yokhazikika.
Car T Cell Therapy: Chiyembekezo Chatsopano cha Chithandizo cha Khansa
CAR-T cell therapy mosakayikira ndi chiyembekezo chatsopano chotsogolera tsogolo la biomedicine ya khansa.Pamasitepe onse opanga ma cell a CAR-T, kuthekera ndi kuchuluka kwa maselo kuyenera kutsimikiziridwa bwino.
The Countstar Rigel yavomerezedwa ndi makampani ambiri omwe akutsogolera chithandizo cha cell cha CAR-T, titha kuthandiza makasitomala athu kuti apange dongosolo lokhazikika, lodalirika la cell, kuwunika koyenera.
