Mesenchymal stem cell ndi kagawo kakang'ono ka pluripotent stem cell omwe amatha kudzipatula ku mesoderm.Ndi mawonekedwe awo odzibwereza okha komanso kusiyanitsa njira zambiri, ali ndi kuthekera kwakukulu kwamankhwala osiyanasiyana azachipatala.Maselo a mesenchymal stem ali ndi phenotype yapadera ya chitetezo chamthupi komanso mphamvu ya chitetezo chamthupi.Chifukwa chake, ma cell stem cell a mesenchymal amagwiritsidwa ntchito kale kwambiri pakuyika ma cell cell, uinjiniya wa minofu ndi kuyika ziwalo.Ndipo kupitilira izi, amagwiritsidwa ntchito ngati chida choyenera muumisiri wa minofu ngati ma cell a seeder pamayesero oyambira komanso azachipatala.
The Countstar Rigel imatha kuyang'anira ndende, kuthekera, kusanthula kwa apoptosis ndi mawonekedwe a phenotype (ndi kusintha kwawo) panthawi yopanga ndi kusiyanitsa ma cell tsinde awa.The Countstar Rigel ilinso ndi mwayi wopeza zidziwitso zowonjezera za morphological, zoperekedwa ndi malo owala osatha komanso kujambula kwazithunzi zozikidwa pa fluorescence panthawi yonse yowunikira kuwunika kwa ma cell.The Countstar Rigelo imapereka njira yachangu, yotsogola komanso yodalirika yowongolera ma cell tsinde.
Kuwunika Kutheka kwa MSCs mu Regenerative Medicine
Chithunzi 1 Kuyang'anira kuthekera ndi kuchuluka kwa ma cell a mesenchymal stem cell (MSCs) kuti agwiritsidwe ntchito pochiza ma cell.
Stem Cell ndi imodzi mwazinthu zomwe zimathandizira kwambiri pakuchiritsa kwa ma cell.Kuyambira pakukolola kwa MSC mpaka kuchiza, ndikofunikira kuti ma cell stem cell azikhala ndi mphamvu panthawi yonse yopanga ma cell cell (Chithunzi 1).Countstar's stem cell counter imayang'anira kuthekera kwa ma cell cell ndi kukhazikika kuti itenge gawo lofunikira pakuwongolera khalidwe.
Kuwunika Kusintha kwa MSC Morphological pambuyo pa Maulendo
M'mimba mwake ndi kuphatikiza zidatsimikiziridwanso ndi Countstar Rigel.Makulidwe a ma AdMSC adasinthidwa kwambiri pambuyo pa mayendedwe poyerekeza ndi asanayendetse.Kukula kwake kusanachitike mayendedwe kunali 19µm, koma kudakwera mpaka 21µm pambuyo pa mayendedwe.Kuphatikizika kwa zisanachitike zoyendera kunali 20%, koma kudakwera mpaka 25% pambuyo pamayendedwe.Kuchokera pazithunzi zomwe zidajambulidwa ndi Countstar Rigel, phenotype ya AdMSCs idasinthidwa kwambiri pambuyo pamayendedwe.Zotsatira zawonetsedwa mu Chithunzi 3.
Kuzindikiritsa ma AdMSC mu Cell Phenotype
Pakadali pano njira zoyeserera zoyeserera zotsimikizira zamtundu wa ma MSC omwe amawunikidwa zalembedwa m'mawu a International Society for Cellular Therapy (ISCT), omwe adafotokozedwa kale mu 2006.
Kuzindikira Mofulumira kwa Apoptosis mu MSCs ndi FITC Conjugated Annexin-V ndi 7-ADD Introduction
Cell Apoptosis imatha kudziwika ndi FITC conjugated annexin-V ndi 7-ADD.PS nthawi zambiri imapezeka papepala la intracellular la plasma membrane m'maselo athanzi, koma kumayambiriro kwa apoptosis, membrane asymmetry imatayika ndipo PS imasunthira ku kapepala kakunja.
Chithunzi 6 Kuzindikira kwa Apoptosis mu MSCs ndi Countstar Rigel
A. Kuyang'ana kowonekera kwa chithunzi cha fluorescence cha Detection of Apoptosis mu MSCs
B. Scatter plots of Apoptosis in MSCs by FCS express
C. Peresenti ya chiwerengero cha maselo kutengera % yachibadwa, % apoptotic, ndi % necrotic/ma cell a apoptotic omwe achedwa kwambiri.
