Kusanthula kwa Immuno-phenotyping ndikuyesa komwe kumachitika m'magawo ofufuza okhudzana ndi ma cell kuti azindikire matenda osiyanasiyana (matenda a autoimmune, matenda a immunodeficiency, chotupa, hemostasis, matenda osagwirizana, ndi zina zambiri) ndi matenda.Amagwiritsidwanso ntchito kuyesa mtundu wa cell mu kafukufuku wama cell osiyanasiyana.Flow cytometry ndi microscope ya fluorescence ndi njira zowunikira pafupipafupi m'mabungwe ofufuza za matenda a cell omwe amagwiritsidwa ntchito pa immuno-phenotyping.Koma njira zowunikirazi zitha kupereka zithunzi kapena mndandanda wazinthu, zokha, zomwe sizingakwaniritse zofunikira zovomerezeka za oyang'anira.
M Dominici el, Cytotherapy (2006) Vol.8, No. 4, 315-317
Kuzindikiritsa Immuno-phenotype ya AdMSCs
Ma immunophenotype a AdMSC adatsimikiziridwa ndi Countstar FL, AdMSCs adalumikizidwa ndi ma antibody osiyanasiyana motsatana (CD29, CD34, CD45, CD56, CD73, CD105, ndi HLADR).Njira yogwiritsira ntchito mtundu wa chizindikiro idapangidwa pokhazikitsa njira yobiriwira kuti iwonetse PE fluorescence, kuphatikiza gawo lowala.Gawo lofotokozera zazithunzi zowala linagwiritsidwa ntchito ngati chigoba kuti muyese chizindikiro cha PE fluorescence.Zotsatira za CD105 zidawonetsedwa (Chithunzi 1).
Chithunzi 1 Kuzindikiritsa Immuno-phenotype ya AdMSCs.A. Bright Field ndi Fluorescence Image of AdMSCs;B. CD Marker Detection of AdMSCs ndi Countstar FL
Kuwongolera kwabwino kwa ma MSC - kutsimikizira zotsatira za selo lililonse
Chithunzi 2 A: Zotsatira za Countstar FL zidawonetsedwa mu FCS Express 5plus, kuwonetsa kuchuluka kwa CD105, ndikuwonetsa ma cell single cell.B: Kulowera kumanja kumanja, zithunzi za tebulo limodzi la cell zimawonetsa ma cell omwe ali ndi CD105.C: Kulowera kumanzere kumanzere, zithunzi za tebulo la maselo amodzi zimasonyeza maselo omwe ali ndi CD105 yochepa.
Kusintha kwa Phenotypical pa Transport
Chithunzi 3. A: Kusanthula kachulukidwe ka kuchuluka kwa CD105 mu zitsanzo zosiyanasiyana ndi FCS Express 5 plus software.B: Zithunzi zapamwamba zimapereka chidziwitso chowonjezera cha morphological.C: Zotsatira zovomerezeka ndi tizithunzi za selo limodzi lililonse, zida za pulogalamu ya FCS zidagawa ma cell kukhala osiyana
