Kuwerengera kwa AOPI Dual-fluoresces ndi mtundu woyeserera womwe umagwiritsidwa ntchito pozindikira kuchuluka kwa ma cell ndi kuthekera kwake.Njira yothetsera vutoli ndi kuphatikiza kwa acridine lalanje (dontho lobiriwira la fulorosenti la nucleic acid) ndi propidium iodide (dontho lofiira la fulorosenti la nucleic acid).Propidium iodide (PI) ndi utoto wochotsa nembanemba womwe umangolowa m'maselo okhala ndi nembanemba wosokonekera, pomwe acridine orange amatha kulowa m'maselo onse mwa anthu.Mitundu yonse iwiri ikakhala mu phata, propidium iodide imayambitsa kuchepa kwa acridine orange fluorescence ndi fluorescence resonance energy transfer (FRET).Chotsatira chake, maselo a nucleated omwe ali ndi nembanemba osasunthika amadetsa zobiriwira za fulorosenti ndipo amawerengedwa kuti ndi amoyo, pamene maselo a nucleated omwe ali ndi nembanemba yowonongeka amangowononga zofiira za fulorosenti ndipo amawerengedwa ngati akufa pogwiritsa ntchito Countstar® FL system.Zinthu zopanda ma nucleated monga maselo ofiira a magazi, mapulateleti ndi zinyalala sizimasungunuka ndipo zimanyalanyazidwa ndi pulogalamu ya Countstar® FL.
Njira ya Stem Cell Therapy
Chithunzi 4 Kuyang'anira kuthekera ndi kuchuluka kwa maselo a mesenchymal stem cell (MSCs) kuti agwiritsidwe ntchito pochiza ma cell.
Tsimikizirani kuthekera kwa MSC ndi AO/PI ndi Trypan Blue assay
Chithunzi 2. A. Chithunzi cha MSC chodetsedwa ndi AO/PI ndi Trypan Blue;2. Kuyerekeza zotsatira za AO/PI ndi Trypan blue musanayende komanso pambuyo.
Ma cell refractive index akusintha, madontho a Trypan Blue sanali odziwikiratu, ndizovuta kudziwa momwe zingakwaniritsire zoyendera.Ngakhale kuti mitundu iwiri ya fluorescence imalola kuti ma cell amoyo ndi akufa akhale odetsedwa, kutulutsa mphamvu zolondola kumabweretsa ngakhale pamaso pa zinyalala, mapulateleti, ndi maselo ofiira a magazi.
