Maseru eMammalian anoshandiswa zvakanyanya muBiopharmaceuticals, senge masoja ekudzivirira chirwere, vaccine, peptides uye yechipiri metabolites anogadzirwa ne bioprocessing nemasero emammalian.Munguva yemaitiro ese kubva kune antibody R&D kusvika mukugadzirwa, kune akawanda matanho anofanirwa kuita sero-based assay kuti uongorore maitiro kana kudzora kwemhando.Zvakadai sekuzara kwesero uye kugona kunotsanangura chimiro chesero tsika.Zvakare nekutapurirana kwesero, hukama hwe antibody hunosarudza padanho resero.Countstar zviridzwa ndiwo mufananidzo wakavakirwa cytometry, unogona kubatsira kutarisa kubva kuR&D kuenda kumagadzirirwo ekugadzira uye kuona kuberekana uye kuenderana.
Cell Count uye Viability by Trypan Blue Staining Principle
Kutarisa uye kuongorora masero etsika nemamiriro-e-the-art mhinduro.Kuvimbika uye kunoshanda kutarisa kwakakosha pakugadzirisa goho uye mhando yechigadzirwa sezvo kunyangwe shanduko diki mumaparamita ebioprocess inogona kukanganisa kuita kwesero rako tsika.Kuverengera kwesero uye kushanda ndiwo akanyanya kukosha paramita, Countstar Altair inopa yakanyanyisa hungwaru uye inoteedzera zvizere cGMP mhinduro yeizvi.
Iyo Countstar Altair yakagadzirwa zvichibva pane yakasarudzika Trypan Blue musimboti, ichibatanidza iyo yepamusoro "fix focus" Optical imaging bhenji, yakanyanya yepamusoro masero ekuzivikanwa matekinoroji, uye software algorithms.Gonesa kuti uwane ruzivo rwekuseswa kwesero, kugona, chiyero chekuunganidza, kutenderera, uye kugovera kwedhayamita nekumhanya kumwe.
Viability uye GFP Transfection Determination muMasero
Munguva yebioprocess, GFP inowanzoshandiswa kusanganisa neprotein recombinant sechiratidzo.Sarudza iyo GFP fluorescent inogona kuratidza chinangwa cheprotein kutaura.Countstar Rigel inopa inokurumidza uye yakapusa bvunzo yekuyedza kutapurirana kweGFP pamwe nekuita.Masero akasvibiswa nePropidium iodide (PI) uye Hoechst 33342 kutsanangura huwandu hwesero akafa uye huwandu hwesero.Countstar Rigel inopa inokurumidza, yehuwandu nzira yekuongorora GFP kutaura kunyatsoita uye kugona panguva imwe chete.
Masero anowanikwa achishandisa Hoechst 33342 (blue) uye chikamu cheGFP chinoratidza maseru (girinhi) chinogona kutariswa zviri nyore.Nonviable cell yakasvibiswa nepropidium iodide (PI; tsvuku).
Kubatana kwekuonekwa kweantibody paCountstar Rigel
Diluted antibody muzvikamu zvakasiyana, ndokuzoiswa nemasero.Mhedzisiro yacho yakawanikwa kubva kuCountstar Rigel (zvese mufananidzo uye huwandu hwemhedzisiro)
Countstar ndeye GMP-yakagadzirira 21 CFR Chikamu 11
Countstar zviridzwa zvinonyatsoenderana ne21 CFR uye Chikamu 11, iyo IQ/OQ/PQ masevhisi anovimbisa kutonga kwekushanda kwakafanana.Countstar zviridzwa zvakagadzirira kushandiswa muGMP uye 21 CFR chikamu 11 inoenderana marabhoritari.Kudzora kwemushandisi uye nzira dzekuongorora dzinobvumira magwaro akakwana ekushandiswa ane yakajairwa marepoti ePDF.
Zvinyorwa zveIQ/OQ uye zvikamu zvekusimbisa
