Ropa uye masero achangobva kuparadzaniswa ekutanga kana maseru ane tsika anogona kunge aine tsvina, akati wandei marudzi emasero kana zvidimbu zvinokanganisa zvakaita semarara emasero izvo zvinoita kuti zvisaite kuongorora maseru ekufarira.Countstar FL ine dual fluorescence nzira yekuongorora inogona kusabvisa maseru zvimedu, marara uye maartifacts zvimedu pamwe neakaderera zviitiko senge maplatelet, zvichipa mhedzisiro chaiyo.
AO/PI Dual Fluorescence Viability Kuverenga
Acridine orenji (AO) uye Propidium iodide (PI) inyukireya nucleic acid inosunga dhayi.Ongororo iyi haibatanidzi zvimedu zvemasero, marara uye maartifacts particles pamwe chete nezviitiko zvakaderera senge tsvuku yeropa, zvichipa mhedzisiro chaiyo.Mukupedzisa, iyo Countstar system inogona kushandiswa kune yega nhanho yesero kugadzira maitiro.
WBCs muRopa Rakazara
Mufananidzo 2 Yese ropa remuenzaniso mufananidzo wakatorwa naCountstar Rigel
Kuongorora maWBCs muropa rose ongororo yenguva dzose mukiriniki lab kana mubhangi reropa.Iko kusungirirwa uye kushanda kweWBCs indekisi yakakosha sekutonga kwemhando yekuchengetedza ropa.
Iyo Countstar Rigel ine AO/PI nzira inokwanisa kunyatsosiyanisa mhenyu uye akafa mamiriro emasero.Iyo Rigel inogona zvakare kuita iyo WBC kuverenga nemazvo asi isingasanganisire kukanganiswa kwemasero matsvuku eropa.
Kuverenga uye Kubudirira kwePBMC
Mufananidzo 3 Bright Field uye Fluorescence mifananidzo yePBMC Yakatorwa naCountstar Rigel
AOPI Dual-fluoresces kuverenga imhando yeassay inoshandiswa kuona kusungwa kwesero uye kushanda.Nekuda kweizvozvo, maseru ane nucleated ane intact membranes anoshatisa fluorescent green uye anoverengerwa semhenyu, nepo nucleated maseru ane mamembres akakanganiswa anongosvibisa fluorescent tsvuku uye anoverengwa seakafa kana uchishandisa Countstar Rigel system.Zvisiri-nucleated zvinhu zvakaita semasero matsvuku eropa, maplatelet nemarara haaite fluoresce uye anofuratirwa neCountstar Rigel software.