Mesenchymal stem masero inhengo yepluripotent stem masero anogona kuparadzaniswa kubva kumesoderm.Nekuzvidzokorora kwavo patsva uye maitiro akasiyana-siyana ekusiyanisa, vane mukana wepamusoro wekurapa kwakasiyana-siyana mukurapa.Mesenchymal stem masero ane yakasarudzika immune phenotype uye immune regulation kugona.Naizvozvo, mesenchymal stem masero atove kushandiswa zvakanyanya mu stem cell transplantation, tishu engineering uye organ transplantation.Uye Kupfuura izvi maapplication, anoshandiswa sechishandiso chakakodzera muinjiniya yetishu seseder cell munhevedzano yezvidzidzo zvekutanga uye zvekiriniki.
Iyo Countstar Rigel inogona kutarisa kutariswa, kugona, apoptosis kuongororwa uye phenotype maitiro (uye shanduko dzadzo) panguva yekugadzira uye kusiyanisa kweaya stem maseru.Iyo Countstar Rigel ine zvakare mukana wekuwana yekuwedzera morphological ruzivo, yakapihwa nekusingaperi inopenya munda uye fluorescence-yakavakirwa mufananidzo kurekodha panguva yese yekuongororwa kwemhando yesero.Iyo Countstar Rigelo inopa inokurumidza, yakaomesesa uye yakavimbika nzira yemhando yekutonga kwe stem maseru.
Kuongorora Kubudirira kweMSCs muRegenerative Medicine
Mufananidzo 1 Kutariswa kwekuita uye kuverengerwa kwesero kwe mesenchymal stem masero (MSCs) ekushandiswa mumasero ekurapa.
Stem Cell ndeimwe yeanonyanya kuvimbisa marapirwo mu regenerative cell therapies.Kubva pakukohwewa kweMSC kusvika pakurapwa, zvakakosha kuchengetedza kusimba kwesero repamusoro panguva dzese nhanho dzekugadzira masero (Mufananidzo 1).Countstar's stem cell counter inotarisisa stem cell kuita uye kutarisisa kuita basa rakakosha mukutonga kwemhando.
Kutarisa MSC Morphological Shanduko mushure meKutakurwa
Iyo dhayamita uye kuunganidzwa kwakatemwa zvakare na Countstar Rigel.Iyo dhayamita yeAdMSCs yakashandurwa zvakanyanya mushure mekufambisa kana ichienzaniswa nepamberi pekutakura.Iyo dhayamita yepamberi yekutakura yaive 19µm, asi yakawedzera kusvika 21µm mushure mekufambisa.Iko kuunganidzwa kwepamberi pekutakura yaive 20%, asi yakawedzera kusvika 25% mushure mekufambisa.Kubva pamifananidzo yakatorwa naCountstar Rigel, iyo phenotype yeAdMSCs yakashandurwa zvakanyanya mushure mekufambisa.Zvigumisiro zvakaratidzwa mumufananidzo 3.
Kuzivikanwa kweAdMSCs muCell Phenotype
Parizvino iwo mashoma akajairwa bvunzo maitiro ekusimbiswa kwemhando yeanotariswa MSCs akanyorwa mukutaura kweInternational Society for Cellular Therapy (ISCT), yakatsanangurwa kare muna 2006.
Kukurumidza Kuonekwa kweApoptosis muMSCs ine FITC Conjugated Annexin-V uye 7-ADD Nhanganyaya.
Cell Apoptosis inogona kuonekwa neFITC conjugated annexin-V uye 7-ADD.PS inongowanikwa chete pane intracellular leaflet yeplasma membrane mumaseru ane hutano, asi panguva yekutanga apoptosis, membrane asymmetry inorasika uye PS inotamira kune yekunze pepa.
Mufananidzo 6 Kuonekwa kweApoptosis muMSCs naCountstar Rigel
A. Kutariswa kunoonekwa kwemufananidzo wefluorescence weDetection of Apoptosis muMSCs
B. Kuparadzira zvirongwa zveApoptosis muMSCs neFCS express
C. Mazana ehuwandu hwemasero kubva pa% normal, % apoptotic, uye % necrotic / very late-stage apoptotic cells.
