AOPI Dual-fluoresces kuverenga imhando yeassay inoshandiswa kuona kusungwa kwesero uye kushanda.Mhinduro isanganiswa yeacridine orange (iyo green-fluorescent nucleic acid stain) uye propidium iodide (iyo red-fluorescent nucleic acid stain).Propidium iodide (PI) idhayi yekusarudzika membrane inongopinda mumasero ane membrane yakakanganiswa, nepo acridine orenji inokwanisa kupinda mumasero ese muhuwandu.Kana madhayi ese ari maviri ari munucleus, propidium iodide inokonzera kuderedzwa kweacridine orange fluorescence nefluorescence resonance energy transfer (FRET).Nekuda kweizvozvo, masero ane nucleated ane intact membranes anoshatisa fluorescent green uye anoverengerwa semhenyu, nepo nucleated maseru ane membrane akakanganiswa anongosvibisa fluorescent tsvuku uye anoverengwa seakafa kana uchishandisa Countstar® FL system.Zvinhu zvisina nucleated zvakaita semasero matsvuku eropa, maplatelet nemarara haaite fluoresce uye anofuratirwa neCountstar® FL software.
Maitiro eStem Cell Therapy
Mufananidzo 4 Kutariswa kwekuita uye kuverengerwa kwesero kwe mesenchymal stem masero (MSCs) kuti ashandiswe mumasero ekurapa.
Sarudza kuita kweMSC neAO/PI uye Trypan Blue assay
Mufananidzo 2. A. Mufananidzo weMSC wakasvibiswa neAO/PI uye Trypan Blue;2. Kuenzanisa kweAO/PI uye Trypan blue mhedzisiro pamberi uye mushure mekutakura.
Iyo cell refractive index inoshanduka, Trypan Blue staining yanga isiri pachena, zvakaoma kuona kugona mushure mekufambisa.Nepo huviri-ruvara fluorescence inobvumira kusvibiswa kwemhenyu uye akafa nucleated maseru, ichigadzira iyo chaiyo inogoneka inoguma kunyangwe mukuvapo kwemarara, maplatelet, uye masero matsvuku eropa.
