Kuedza Protocol
Iyo cytotoxicity % inoverengerwa neiyo equation iri pazasi.
Cytotoxicity% = (Kurarama kuverenga kwekutonga - Kurarama kuverenga kwekurapwa) / Hupenyu kuverenga kwekutonga × 100
Nekuisa mazita anonangwa bundu maseru neasina-chepfu, asiri-radioactive calcein AM kana transfect neGFP, tinogona kutarisa kuurayiwa kwemaseru bundu neCAR-T maseru.Nepo maseru egomarara anorarama achizonyorwa negirinhi calcein AM kana GFP, masero akafa haakwanise kuchengetedza dhayi yegirinhi.Hoechst 33342 inoshandiswa kusvibisa masero ese (ese maT masero uye bundu masero), neimwe nzira, inonangwa bundu masero anogona kusvibiswa ne membrane akasungwa calcein AM, PI inoshandiswa kusvibisa masero akafa (ese maT masero uye bundu masero).Iyi nzira yekusvibisa inobvumira kusarura kwemasero akasiyana.
E: T Ratio inotsamira Cytotoxicity yeK562
Muenzaniso Hoechst 33342, CFSE, PI fluorescent mifananidzo ndiyo K562 inotariswa maseru pa t = maawa matatu.
Mifananidzo yakakonzerwa nefluorescent yakaratidza kuwedzera kweHoechst+CFSE+PI+ Target masero seE: T chiyero chakawedzera.
