Liphekolo tsa liphatsa tsa lefutso tsa Biologics le AAV li fumana karolo e eketsehileng ea 'maraka bakeng sa kalafo ea mafu.Leha ho le joalo, ho hlahisa lisele tse matla le tse sebetsang hantle tsa mammalian bakeng sa tlhahiso ea tsona ke phephetso 'me hangata ho hloka sebopeho se pharaletseng sa lisele.Ho latela nalane, cytometer ea phallo e ne e sebelisoa litekong tsena tse thehiloeng ho lisele.Leha ho le joalo, cytometer ea phallo e batla e theko e boima 'me e kenyelletsa koetliso e pharaletseng bakeng sa ts'ebetso le tlhokomelo.Morao tjena, ka keketseho ea bokhoni ba lik'homphieutha le lisebelisoa tsa lik'hamera tsa boleng bo phahameng, cytometry e thehiloeng ho setšoantšo e nchafalitsoe ho fana ka mokhoa o nepahetseng le o theko e tlaase bakeng sa nts'etsopele ea lisele tsa lisele.Mosebetsing ona, re hlalositse phallo ea tšebetso ea lisele tsa lisele tse kenyelletsang cytometer e thehiloeng ho setšoantšo, e leng Countstar Rigel, bakeng sa tlhahlobo ea ts'ebetso ea phetisetso le tlhahlobo e tsitsitseng ea letamo ka ho sebelisa lisele tsa CHO le HEK293 tse hlalosang antibody le rAAV vector, ka ho latellana.Lithutong tse peli tsa mohlala, re bonts'itse:
1. Countstar Rigel e fane ka ho nepahala ho ts'oanang ho lemoha ho phalla ha cytometry.
2. Tlhahlobo ea letamo e thehiloeng ho Countstar Rigel e ka thusa ho fumana sehlopha se lakatsehang sa single-cell cloning (SCC).
3. Countstar Rigel e kenyellelitse sethala sa nts'etsopele ea lisele tse fihletsoeng 2.5 g/L mAb titer.
Hape re buisane ka monyetla oa ho sebelisa Countstar joalo ka karolo e 'ngoe ea sepheo sa ntlafatso se thehiloeng ho rAAV DoE.
Tobetsa mona bakeng sa lintlha tse ling
