Likarolo tsa sehlahisoa
Theknoloji e ncha ea Optical Multiplication
Theknoloji e ikhethang ea ho atametsa e thusa basebelisi ho sekaseka lisele ka mefuta e mengata e fapaneng ea li-diameter
Ha u sebelisa tšimo e khanyang ea BioApp templates ho Countstar Mira, buka ea Zooming Technology e thusa mosebeletsi ho tseba ka nepo lintho tsa cellular ka har'a sebaka sa diamater ho tloha ho 1.0µm ho ea ho 180.0µm.Litšoantšo tse nkiloeng li bonts'a lintlha tsa lisele tse le 'ngoe.Sena se holisa mefuta e mengata ea lits'ebetso esita le linthong tsa cellular, tse neng li ke ke tsa hlahlojoa ka nepo nakong e fetileng.
| Mehlala ea mela e tloaelehileng ea lisele e hokahaneng le magnification a khethiloeng 5x, 6.6x, le 8x |
| Khatiso ea Diameter e fapaneng | 5x | 6.6x | 8x |
| >10µm | 5-10 µm | 1-5 µm |
| Ho bala | ✓ | ✓ | ✓ |
| Vability | ✓ | ✓ | ✓ |
| Mofuta oa Lisele | - MCF7
- HEK293
- CHO
- MSC
- RAW264.7
| - Sele ea Tšireletso ea 'mele
- Tomoso ea biri
- Lisele tse embryo tsa Zebrafish
| - Pichia Pastoris
- Chlorella vulgaris (FACHB-8)
- Escherichia
|
Li-algorithms tse tsoelang pele tsa AI tse thehiloeng ho Image Analysis
The Countstar Mira FL e sebelisa melemo ea Artifical Intelligence ho nts'etsapele mekhoa ea ho ithuta ea ho ithuta.Ba khona ho khetholla le ho sekaseka litšobotsi tse ngata tsa lisele.Ho kopanngoa ha likarolo tsa sebopeho sa lisele ho lumella tlhahlobo e nepahetseng haholo le e ka hlahisoang hape ea boemo ba potoloho ea lisele le / kapa ho fana ka lintlha tse mabapi le kamano pakeng tsa phetoho ea morphology ea lisele, ho thehoa ha lihlopha tsa lisele (kakaretso, li-spheroids tse nyenyane) le maemo a amehang.
Ho ngola liphetho tsa li-Mesenchymal Stem Cells (MSC; 5x mangification) ka sebopeho se sa tloaelehang
- Li-circles tse tala li tšoaea lisele tse phelang
- Li-circles tse khubelu li tšoaea lisele tse shoeleng
- Lisele tse tšoeu tse kopaneng
RAW264.7 cell line ke e nyane ebile e phutheha habonolo.Algorithm ea Countstar AI e ka khetholla lisele tse ka har'a li-clumps le ho bala
- Li-circles tse tala li tšoaea lisele tse phelang
- Li-circles tse khubelu li tšoaea lisele tse shoeleng
- Lisele tse tšoeu tse kopaneng
Boholo bo sa leka-lekaneng ba lisele tsa embryonic tsa zebrafish (6.6X magnification
- Li-circles tse tala li tšoaea lisele tse phelang
- Li-circles tse khubelu li tšoaea lisele tse shoeleng
- Lisele tse tšoeu tse kopaneng
Moralo oa Intuitive Graphical User Interface (GUI).
GUI e hlophisitsoeng e hlakileng e lumella ho etsa liteko tse sebetsang hantle le tse phutholohileng
- Laeborari e pharalletseng e nang le mefuta ea lisele tse seng e setiloe le BioApps (liprothokholo tsa template ea assay).Tlanya e le 'ngoe feela ho BioApp,' me tlhahlobo e ka qala.
- GUI e bonolo ea basebelisi e etsa hore ho be bonolo ho fetola lipakeng tsa likhetho tse fapaneng tsa menyetla le ho netefatsa boiphihlelo bo botle ba tlhahlobo
- Li-module tse hlophisitsoeng tse hlophisitsoeng hantle li tšehetsa mosebelisi ho etsa liteko tsa letsatsi le letsatsi
Khetha BioApp, kenya Mohlala oa ID, 'me u qale ho etsa tlhahlobo
128 GB ea bokhoni ba ho boloka boitsebiso bo ka hare, bo lekaneng ho boloka hoo e ka bang.Liphetho tsa tlhahlobo ea 50,000 ho Countstar (R) Mira.Bakeng sa ho fihlella kapele, data e batloang e ka khethoa ka likhetho tse fapaneng tsa ho batla.
Karolo ea bohlokoa ea ho boloka nako, ke sebali sa ho hlapolla se ka khutlisoang.E tla fana ka palo e nepahetseng ea sampole ea lisele tse hlapollang le tsa mantlha, hang ha khatello ea ho qetela ea lisele le bophahamo ba molumo o kentsoeng.Sena se etsa hore ho fetisoa ha lisele ho li-subcultures tsa bona ho phutholohe.
Likarolo tse ngata tsa ts'ebeliso
Likarolo tsa tlhahlobo ea Countstar Mira li ts'ehetsa mosebelisi ho utloisisa liphetoho tse matla ka har'a setso sa lisele le ho thusa ho ntlafatsa maemo a bona a ntseng a hola.
Software e tsoetseng pele, e thehiloeng ho AI ea ho lemoha litšoantšo ea Countstar Mira e khona ho fana ka litekanyo tse ngata.Ntle le liphetho tse tloaelehileng tsa mahloriso a lisele le boemo ba ts'ebetso, kabo ea boholo ba sele, ho ka etsahala hore ho be le lihlopha tsa lisele, matla a lekanyelitsoeng a fluorescence ea sele e 'ngoe le e' ngoe, sebopeho sa lekhalo la kholo, le lintlha tsa bona tsa kantle tsa morphology ke lintlha tsa bohlokoa ho lekola boleng ba 'nete. boemo ba setso sa sele.Li-graph tsa li-curve tsa kholo, kabo ea bophara le li-histogram tsa matla a fluorescence, tlhahlobo ea sele e le 'ngoe ka har'a likakaretso le qeto ea paramente ea compactness ea sele e thusa mosebelisi ho utloisisa hamolemo lits'ebetso tse matla ka har'a setso se hlahlobiloeng ho tloha qalong ho isa qetellong ea ts'ebetso.
Histogram
Relative Fluorescence Intensity (RFI) histogram ea kabo
Diameter kabo histogram
Mokokotlo wa kgolo
Lekola litšoantšo le Liphetho
Setšoantšo sa curve ea kholo
Sesebelisoa sa Sehlahisoa
AO/PI dual fluorescence cell density le liteko tsa ho sebetsa
The dual-fluorescence AO/PI staining method e theilwe hodima molaomotheo, wa hore didae ka bobedi, Acridine Orange (AO) le Propidium Iodide (PI), di hokahana dipakeng tsa nucleic acid tsa chromosome khubung ya sele.Le ha AO e khona ho kenella ka har'a li-membrane tsa nucleus ka nako efe kapa efe le ho silafatsa DNA, PI e ka fetisa feela lera le senyehileng la khubu ea sele e shoang (e shoeleng).AO e bokelletsoeng ka har'a nucleus ea sele e hlahisa leseli le letala ka holimo ho 525nm, haeba e thabisoa ke 480nm, PI e romela leseli le khubelu ka amplitude ea 615nm, ha e thabetse 525nm.Phetolelo ea FRET (Foerster Resonance Energy Transfer) e tiisa, hore lets'oao le ntšitsoeng la AO ho 525nm le kenngoa ka pel'a dae ea PI ho qoba ho tsoa habeli le ho qhalana.Motsoako ona o khethehileng oa dae oa AO/PI o lumella ho sefa ka ho khetheha nucleus e nang le lisele ka pel'a li-acaryote tse kang erythrocyte.
Lintlha tsa Countstar Mira FL li bonts'itse linearity e ntle bakeng sa phokotso ea gradient ea lisele tsa HEK293
Tlhahlobo ea katleho ea phetisetso ea GFP/RFP
Bokhoni ba phetisetso ke index ea bohlokoa ntlafatsong le ntlafatsong ea lisele, tokisong ea vector ea vaerase, le bakeng sa tlhahlobo ea tlhahiso ea lihlahisoa lits'ebetsong tsa Biopharma.E se e le teko e atisang ho etsoa ea ho fumana ka potlako se ka har'a protheine e shebiloeng ka har'a sele.Mekhoeng e fapaneng ea kalafo ea liphatsa tsa lefutso, ke sesebelisoa sa bohlokoa ho laola ts'ebetso ea phetiso ea liphatsa tsa lefutso tse lakatsehang.
Countstar Mira ha e fane feela ka liphello tse nepahetseng le tse nepahetseng, ha li bapisoa le cytometry ea phallo, ho phaella moo mohlahlobi o fana ka litšoantšo e le bopaki ba bopaki.Ntle le sena, e nolofatsa haholo le ho potlakisa tlhahlobo ho nolofatsa nts'etsopele ea ts'ebetso ea nts'etsopele le tlhahiso.
Letoto la litšoantšo, le fumanoeng ke Countstar(R) Mira, le bonts'a maemo a ntseng a eketseha a ts'ebetso ea phetisetso (ho tloha ka letsohong le letšehali ho ea ho le letona) ea lisele tse fetotsoeng ka liphatsa tsa lefutso (HEK 293 cell line; e hlalosang GFP maemong a fapaneng)
Liphello tsa litekanyo tsa ho bapisa, tse entsoeng ka B / C CytoFLEX, tse tiisang lintlha tsa ts'ebetso ea GFP ea phetisetso ea lisele tsa HEK 293 tse fetotsoeng, tse hlahlobiloeng ho Countstar Mira.
Tlhahlobo ea ts'ebetso ea Trypan Blue e phatlalalitsoeng ka bophara
Teko ea khethollo ea Trypan Blue e ntse e le e 'ngoe ea mekhoa e sebelisoang haholo le e tšepahalang ea ho tseba palo ea lisele tse shoeleng (tse shoang) ka har'a setso sa lisele tse emisitsoeng.Lisele tse sebetsang tse nang le sebopeho sa membrane e kantle ea lisele li tla leleka Trypan Blue hore e se ke ea phunyeletsa lera.Haeba ho ka etsahala, lera la sele lea lutla ka lebaka la tsoelo-pele ea lefu la lisele tsa eona, Trypan Blue e ka fetisa mokoallo oa lera, e bokellana ka har'a plasma ea sele ebe e senya sele e putsoa.Phapang ena ea optical e ka sebelisoa ho khetholla lisele tse phelang tse sa pheleng ho tsoa liseleng tse shoeleng ka mokhoa oa ho lemoha litšoantšo tsa Countstar Mira FL.
- Litšoantšo tsa tse tharo, li-cell line tsa Trypan Blue, tse fumanoeng ka Countstar (R) Mira FL ka mokhoa o khanyang oa tšimo.
- Liphetho tsa "dilution gradient" tsa HEK 293 series
