Tlhahlobo ea Immuno-phenotyping ke teko e tloaelehileng e etsoang mafapheng a lipatlisiso a amanang le lisele ho hlahloba mafu a fapaneng (lefu la autoimmune, lefu la immunodeficiency, lefu la hlahala, hemostasis, mafu a allergic, le tse ling tse ngata) le lefu la mafu.E boetse e sebelisoa ho lekola boleng ba lisele lipatlisisong tse fapaneng tsa mafu a lisele.Flow cytometry le microscope ea fluorescence ke mekhoa e tloaelehileng ea tlhahlobo litsing tsa lipatlisiso tsa mafu a lisele tse sebelisoang bakeng sa immuno-phenotyping.Empa mekhoa ena ea ho hlahloba e ka fana ka litšoantšo kapa letoto la lintlha, feela, tse ka 'nang tsa se ke tsa finyella litlhoko tse tiileng tsa tumello ea balaoli ba tsamaiso.
M Dominici el, Cytotherapy (2006) Moq.8, No. 4, 315-317
Ho tsebahatsa Immuno-phenotype ea AdMSCs
The immunophenotype ea AdMSCs e ile ea khethoa ke Countstar FL, AdMSCs e ne e kenngoa ka li-antibody tse fapaneng ka ho latellana (CD29, CD34, CD45, CD56, CD73, CD105, le HLADR).Ts'ebetso ea ts'ebeliso ea mebala ea matšoao e entsoe ka ho beha mocha o Motala ho etsa setšoantšo sa PE fluorescence, hammoho le lebala le khanyang.Karolo ea litšupiso tsa litšoantšo tse khanyang e sebelisitsoe joalo ka maske ho etsa sampole lets'oao la PE fluorescence.Liphetho tsa CD105 li bontšitsoe (Setšoantšo sa 1).
Setšoantšo sa 1 Boitsebiso ba Immuno-phenotype ea AdMSCs.A. Sebaka se Khanyang le Setšoantšo sa Fluorescence sa li-AdMSC;B. CD Marker Detection of AdMSCs by Countstar FL
Taolo ea boleng ba li-MSC - ho netefatsa liphetho bakeng sa sele ka 'ngoe
Setšoantšo sa 2 A: Liphetho tsa Countstar FL li hlahisitsoe ho FCS Express 5plus, ho fumana liperesente tse ntle tsa CD105, le kakaretso ea lisele tse le 'ngoe tsa tafole.B: Keiti e fetotsoeng ka lehlakoreng le letona, litšoantšo tsa tafole e le 'ngoe ea sele e bonts'a lisele tse nang le polelo e phahameng ea CD105.C: Keiti e fetotsoeng ka lehlakoreng le letšehali, litšoantšo tsa tafole e le 'ngoe ea lisele li bonts'a lisele tse nang le polelo e tlase ea CD105.
Phenotypical Liphetoho nakong ea Lipalangoang
Setšoantšo sa 3. A: Tlhahlobo ea palo ea peresente e ntle ea CD105 lisampoleng tse fapaneng ka FCS express 5 plus software.B: Litšoantšo tsa boleng bo holimo li fana ka tlhahisoleseding e eketsehileng ea morphological.C: Liphetho tse netefalitsoeng ka li-thumbnails tsa sele e 'ngoe le e' ngoe, lisebelisoa tsa software tsa FCS li arola lisele ho fapana
