Ho bala ha AOPI Dual-fluoresces ke mofuta oa liteko o sebelisetsoang ho bona hore na lisele li sebetsa joang.Tharollo ke motsoako oa acridine orange (letheba la nucleic acid e tala-fluorescent) le propidium iodide (letheba le khubelu la nucleic nucleic acid).Propidium iodide (PI) ke dae e sa kenyelletsoeng lera e kenang feela liseleng tse nang le li-membrane tse senyehileng, athe acridine orange e khona ho kenella liseleng tsohle ho baahi.Ha lidae ka bobeli li le teng khubung, propidium iodide e baka phokotso ea acridine orange fluorescence ka fluorescence resonance energy transfer (FRET).Ka lebaka leo, lisele tse nang le li-nucleated tse nang le li-membrane tse sa tsitsang li senya botala ba fluorescent 'me li baloa li phela, athe lisele tsa nucleated tse nang le li-membrane tse senyehileng li senya bofubelu ba fluorescent feela' me li baloa li shoele ha li sebelisa tsamaiso ea Countstar® FL.Lintho tse se nang nucleated tse kang lisele tse khubelu tsa mali, li-platelet le litšila ha li na fluoresce 'me li hlokomolohuoa ke software ea Countstar® FL.
Mokhoa oa Therapy ea Stem Cell
Setšoantšo sa 4 Ho lekola ts'ebetso le palo ea lisele tsa mesenchymal stem cell (MSCs) bakeng sa tšebeliso ea kalafo ea lisele.
Etsa qeto ea hore na MSC e sebetsa joang ka AO/PI le Trypan Blue
Setšoantšo sa 2. A. Setšoantšo sa MSC se silafalitsoeng ke AO/PI le Trypan Blue;2. Papiso ea sephetho sa AO/PI le Trypan blue pele le ka morao ho sepalangoang.
Li-index tsa refractive index lia fetoha, "Trypan Blue staining" e ne e sa totobala joalo, ho thata ho tseba hore na ho ka khoneha joang kamora ho tsamaisoa.Le hoja fluorescence ea mebala e 'meli e lumella ho silafatsa lisele tse phelang le tse shoeleng tsa nucleated, ho hlahisa ts'ebetso e nepahetseng ho fella ka boteng ba maloanlahla, liplatelete le lisele tse khubelu tsa mali.
