Unyango lweeseli ngokungathandabuzekiyo lithemba elitsha lokukhokela ikamva le-biomedicine, kodwa ukusetyenziswa kweeseli zomntu kumayeza akusiyo ingcamango entsha.Kwiminyaka embalwa edlulileyo, unyango lweeseli lwenze inkqubela phambili enkulu, kwaye unyango lweeseli ngokwalo alusekho ingqokelela elula yeeseli kunye nokubuyisela umva.Iiseli ngoku zihlala zifuneka ukuba zenziwe nge-bioengineered, ezifana nonyango lweeseli ze-CAR-T.Sijonge ukukubonelela ngezixhobo ezisemgangathweni, ezikumgangatho we-GMP zolawulo lomgangatho weseli.Imveliso ye-Countstar yamkelwe ziinkampani ezininzi ezikhokela unyango lweeseli, sinokunceda umthengi wethu ukuba akhe i-concentration yeseli ezinzileyo, ethembekileyo, inkqubo yokubeka iliso esebenzayo.
Umngeni kwisibalo seeSeli kunye nokusebenza
Ngexesha lawo onke amanyathelo okwenziwa kweeseli ze-CAR-T zeklinikhi, ukusebenza kunye nokubalwa kweeseli kufuneka kumiselwe ngokuchanekileyo.
Iiseli ezisandul' ukwahlukaniswa zeprayimari okanye iiseli ezikhulisiweyo zinokuqulatha ukungcola, iintlobo ezininzi zeeseli okanye amasuntswana aphazamisayo afana nobutyobo lweeseli eziya kwenza ukuba kungenzeki ukuhlalutya iiseli zomdla.
Ukubala kokusebenza kweFluorescence kabini ngu-Countstar Rigel S2
IAcridine orenji (AO) kunye nePropidium iodide (PI) ziidayi ezibophelelayo kwinucleic acid.I-AO inokungena kuzo zombini iiseli ezifileyo neziphilayo kwaye ingcolise iiseli ze-nucleated ukuvelisa i-fluorescence eluhlaza.I-PI inokungcolisa iiseli ze-nucleated ezifileyo kunye ne-membrane edibeneyo kwaye zenze i-fluorescence ebomvu.Uhlalutyo alubandakanyi amaqhekeza eeseli, i-debris kunye ne-artifacts particles kunye neziganeko ezincinci ezifana neeplatelet, zinika umphumo ochanekileyo.Ukuqukumbela, inkqubo ye-Countstar S2 inokusetyenziselwa inyathelo ngalinye lenkqubo yokwenziwa kweeseli.
A: Indlela ye-AO/PI inokwahlula ngokuchanekileyo imeko ephilayo nefileyo yeeseli, kwaye ingangabandakanyi ukuphazamiseka.Ngokuvavanya iisampulu zokuxutywa, indlela ye-dual-fluorescence ibonisa iziphumo ezizinzileyo.
Ukumiselwa kweT / NK Cell Mediated Cytotoxicity
Ngokubhala iiseli zethumba ekujoliswe kuzo nge-non-toxic, non-radioactive calcein AM okanye i-transfect nge-GFP, sinokubeka iliso ekubulaweni kweeseli zethumba ngeeseli ze-CAR-T.Ngelixa iiseli zomhlaza ezijoliswe kuzo ziya kubhalwa nge-calcein eluhlaza i-AM okanye i-GFP, iiseli ezifileyo azikwazi ukugcina idayi eluhlaza.Hoechst 33342 isetyenziselwa ibala zonke iiseli (zombini iiseli T kunye neeseli ithumba), ngenye indlela, iiseli ithumba ekujoliswe kusenokungcoliswa inwebu ebotshwe calcein AM, PI isetyenziselwa ibala iiseli ezifileyo (zombini iiseli T kunye neeseli ithumba).Esi sicwangciso sokucoca sivumela ukucalulwa kweeseli ezahlukeneyo.
Ukubalwa kweeSeli okuhambelanayo kunye noLawulo lweDatha yehlabathi
Ingxaki eqhelekileyo ekubalweni kweeseli eziqhelekileyo ngumahluko wedatha phakathi kwabasebenzisi, amasebe kunye namaziko.Zonke i-Countstar analyzer zibala ngokufanayo kwindawo eyahlukileyo okanye indawo yokuvelisa.Oku kungenxa yokuba kwinkqubo yokulawula umgangatho, isixhobo ngasinye kufuneka silinganiswe kwisixhobo esisemgangathweni.
Ibhanki yedatha ephakathi ivumela umsebenzisi ukuba agcine yonke idatha, njengengxelo yovavanyo lwesixhobo, ingxelo yesampula yeseli kunye ne-tester e-signature, ikhuselekile kwaye isigxina.
Car T Cell Therapy: Ithemba elitsha loNyango loMhlaza
Unyango lweeseli ze-CAR-T ngokungathandabuzekiyo lithemba elitsha lokukhokela ikamva le-biomedicine yomhlaza.Ngexesha lawo onke amanyathelo okwenziwa kweeseli ze-CAR-T zeklinikhi, ukusebenza kunye nokubalwa kweeseli kufuneka kumiselwe ngokuchanekileyo.
I-Countstar Rigel yamkelwe ziinkampani ezininzi ezikhokela unyango lweeseli ze-CAR-T, sinokunceda umthengi wethu ukuba akhe i-concentration yeseli ezinzileyo, ethembekileyo, inkqubo yokubeka iliso esebenzayo.
