Iiseli ze-Mesenchymal stem yi-subset yeeseli ze-pluripotent stem ezinokuthi zibekwe zodwa kwi-mesoderm.Ngokuhlaziya kwabo ukuziphindaphinda kunye neempawu zokwahlula-hlula iindlela ezininzi, banamandla aphezulu onyango olwahlukeneyo kwezamayeza.Iiseli ze-Mesenchymal stem zine-phenotype ekhethekileyo ye-immune kunye nekhono lokulawula i-immune.Ke ngoko, iiseli ze-mesenchymal stem sele zisetyenziswa ngokubanzi kwi-stem cell transplantation, ubunjineli bezicubu kunye nokutshintshwa kwamalungu.Kwaye Ngaphaya kwezi zicelo, zisetyenziswa njengesixhobo esifanelekileyo kubunjineli beethishu njengeeseli zembewu kuthotho lovavanyo olusisiseko kunye nolwekliniki.
I-Countstar Rigel inokubeka iliso kugxininiso, ukusebenza, uhlalutyo lwe-apoptosis kunye neempawu ze-phenotype (kunye neenguqu zabo) ngexesha lokuvelisa kunye nokwahlula kwezi seli ze-stem.I-Countstar Rigel nayo inenzuzo ekufumaneni ulwazi olongezelelweyo lwe-morphological, olubonelelwa yintsimi eqaqambileyo esisigxina kunye nokurekhoda kwemifanekiso esekelwe kwi-fluorescence ngexesha lenkqubo yonke yokujonga umgangatho weseli.I-Countstar Rigelo ibonelela ngendlela ekhawulezayo, ephucukileyo nethembekileyo yolawulo lomgangatho weeseli zesiqu.
Ukubeka iliso kokuSebenza kwee-MSCs kwi-Regenerative Medicine
Umzobo 1 Ukubeka iliso kokusebenza kunye nokubala kweeseli ze-mesenchymal stem cells (MSCs) zokusetyenziswa kwiiseli zonyango.
I-Stem Cell lolona nyango luthembisayo kunyango lweeseli ezivuselelayo.Ukusukela ekuvuneni kwe-MSC ukuya kunyango, kubalulekile ukugcina ukusebenza kweseli ephezulu ngexesha lawo onke amanyathelo emveliso ye-stem cell (Umfanekiso 1).Ikhawuntala ye-stem cell counter ye-Countstar ibeka iliso ekusebenzeni kweeseli ze-stem kunye nokugxila ukuze idlale indima ebalulekileyo kulawulo lomgangatho.
Ukubeka iliso kwi-MSC yoTshintsho lweMophological emva koThutho
Idayamitha kunye nokudityaniswa kwagqitywa ngu-Countstar Rigel.Ubungakanani bee-AdMSCs zatshintshwa kakhulu emva kothutho xa kuthelekiswa nangaphambili kwezothutho.Idayamitha yangaphambi kothutho yayiyi-19µm, kodwa yonyuka yaya kutsho kwi-21µm emva kothutho.Umdibaniso wangaphambili wezothutho wawuyi-20%, kodwa unyuke waya kuma-25% emva kothutho.Ukususela kwimifanekiso ebanjwe ngu-Countstar Rigel, i-phenotype ye-AdMSCs yatshintshwa kakhulu emva kokuthutha.Iziphumo ziboniswe kuMfanekiso 3.
Ukuchongwa kwee-AdMSCs kwi-Cell Phenotype
Okwangoku owona mgangatho usezantsi wovavanyo lweenkqubo zokuqinisekisa umgangatho wee-MSC ezibekwe esweni zidweliswe kwingxelo ye-International Society for Cellular Therapy (ISCT), esele ichazwe ngo-2006.
Ukufunyanwa ngokukhawuleza kwe-Apoptosis kwii-MSC kunye neFITC Conjugated Annexin-V kunye ne-7-ADD Intshayelelo
I-Cell Apoptosis inokufunyanwa nge-FITC conjugated annexin-V kunye ne-7-ADD.I-PS idla ngokufunyanwa kuphela kwiphecana le-intracellular ye-plasma membrane kwiiseli eziphilileyo, kodwa ngexesha le-apoptosis yokuqala, i-membrane asymmetry ilahleka kwaye i-PS idlulisela kwiphecana langaphandle.
Umzobo we-6 Ukufunyanwa kwe-Apoptosis kwii-MSC ngu-Countstar Rigel
A. Ukuhlolwa okubonakalayo komfanekiso we-fluorescence wokufunyanwa kwe-Apoptosis kwii-MSCs
B. Ukusasaza izicwangciso ze-Apoptosis kwi-MSCs yi-FCS express
C. Ipesenti yabantu beeseli ezisekelwe kwi-% eqhelekileyo, i-% ye-apoptotic, kunye ne-% necrotic / iiseli ze-apoptotic zasemva kwexesha kakhulu.
