Ekhaya » NgeBioprocessing

Into esinokuyenza

  • I-Trypan Blue Cell Ukubala
  • Ukusebenza kunye noTshintsho lwe-GFP
  • Antibodies Affinity
Trypan Blue Cell Counting
I-Trypan Blue Cell Ukubala

I-Trypan Blue Cell Ukubala

Ukubeka iliso kunye nokuhlalutya inkcubeko yeseli kunye nezisombululo zanamhlanje.Ukubeka iliso okuthembekileyo nokusebenzayo kubalulekile ekwandiseni isivuno kunye nomgangatho wemveliso kuba notshintsho oluncinci kwiparamitha ye-bioprocess inokuphembelela ukusebenza kwenkcubeko yeeseli zakho.Ukubalwa kweeseli kunye nokusebenza zezona parameters zibaluleke kakhulu, i-Countstar® Altair ibonelela ngobuchule obugqithisileyo kwaye ithobela ngokupheleleyo isisombululo se-cGMP kwezi.

Viability and GFP Transfection
Ukusebenza kunye noTshintsho lwe-GFP

Ngexesha le-bioprocess, i-GFP isoloko isetyenziselwa ukuxubha kunye neprotheni edibeneyo njengesalathisi.Ukumisela i-fluorescent ye-GFP ingabonakalisa inkcazo yeprotheyini ekujoliswe kuyo.I-Countstar Rigel ibonelela ngovavanyo olukhawulezayo nolulula lovavanyo losulelo lwe-GFP kunye nokusebenza.Iiseli zadyojwa ngePropidium iodide (PI) kunye neHoechst 33342 ukuchaza inani leeseli ezifileyo kunye neeseli ezipheleleyo.I-Countstar Rigel ibonelela ngendlela ekhawulezayo, yobungakanani bokuvavanya ukusebenza kakuhle kokubonakaliswa kwe-GFP kunye nokusebenza ngexesha elinye.

Antibodies Affinity
Antibodies Affinity
I-anti-affinity antibodies idla ngokulinganiswa ngu-Elisa okanye i-Biacore, ezi ndlela zinovakalelo kakhulu, kodwa zifumanisa i-antibody kunye neprotheni ecocekileyo, kodwa i-protein ye-conformation yendalo.Sebenzisa indlela ye-cell immunofluorescence, umsebenzisi unokubona ukuhambelana kwe-antibody kunye neprotheni yendalo yendalo.Okwangoku, ubungakanani be-affinity ye-antibody ihlalutywa yi-flow cytometry.I-Countstar Rigel inokubonelela ngendlela ekhawulezayo nelula yokuvavanya ukunxulumana kwe-antibody.
I-Countstar Rigel inokubamba ngokuzenzekelayo umfanekiso kunye nobungakanani bokuqina kwe-fluorescence enokubonisa ukuhambelana kwe-antibody.

Iimveliso ezicetyiswayo

Countstar Altair

Countstar Altair

Yenzelwe ukusetyenziswa kwemihla ngemihla kwiindawo ezilawulwayo ze-cGMP
  • Ikhono lokulawula idatha efihliweyo enamandla
  • Amanqanaba amane okufikelela komsebenzisi kunye nolawulo
  • Imisayino ye-E kunye neefayile zelogi ngokuthobela ngokupheleleyo i-FDA 21 CFR iSigaba se-11
    • Countstar Rigel S2

    I-Countstar Rigel S2

    Fluorescence cell analyzer
  • Idibanisa i-fluorescence ezimbini kunye kunye nomjelo webala elikhanyayo
  • Kwizinto eziphambili zeeseli, iisampuli zegazi zeperipheral, i-CAR-T, NK, kunye neeseli ze-MSC
  • Isiqinisekiso sokwenziwa lula kweemvavanyo
    • Countstar Rigel S3

    Countstar Rigel S3

    Isisombululo esisiNgqongileyo sokubalwa kweeSeli, uCalucalulo lokuSebenza kweeSeli, kunye ne-T / NK yeCell Mediated Cytotoxicity Assays
  • Idibanisa i-fluorescence emithathu kunye nomjelo webala elikhanyayo
  • Inkqubo eqhutywa yi-application, compact, automated cell imaging system
  • Yenzelwe ukwenza lula imisebenzi yesiqhelo yaselebhu
    • Countstar BioTech

    Countstar BioTech

    Uhlalutyi lwakho oluchanekileyo noluthembekileyo ekubekeni iliso kwimveliso yeeseli
  • Hlalutya iisampulu ngokuphindaphindiweyo, vumela isoftware ukubala i-avareji yeesampuli ezahlukeneyo, kwaye uthelekise idatha yohlalutyo olulodwa
  • ukuya kuthi ga kwi-2,000 yeeseli ngaphakathi komfanekiso omnye we-Countstar BioTech
  • Hlalutya ngokuchanekileyo iiseli enye nangaphakathi kwi-aggregate complexes
  • Sebenzisa iiparamitha ezibonelelwe ngokuzenzekelayo ezifana nedayamitha yeseli, ukungqukuva kweseli kunye nesantya sokuhlanganisa
    • Countstar BioMed

    Countstar BioMed

    Umhlalutyi wokujongwa kwe-stem cell, uvavanyo lokuphendula amajoni omzimba, uphando lomhlaza, uphuhliso lonyango olusekwe kwiseli, kunye nohlalutyo lwe-PBMC.
  • Ichonga kwaye ihlele iiseli ezincinci zezilwanyana ezincancisayo ngokuthembekileyo kwindawo eqaqambileyo (i-Trypan Blue engabandakanyi)
  • Inqanaba eliphezulu, i-algorithms yokuqonda imifanekiso ephucukileyo kuquka uhlalutyo olupheleleyo
  • I-cGMP ethobela ulawulo lofikelelo lomsebenzisi, utyikityo lwe-elektroniki, iingxelo zeziphumo, kunye neefayile zenkqubo yokungena
  • Iingxelo zeziphumo zePDF ezilungele wena

    • Izibonelelo eziyeleleneyo

    T/NK Cell Mediated Cytotoxicity Analysis by CFSE, Hoechest 33342 kunye PI

    IProtokholi yoMfuniselo I-cytotoxicity % ibalwa ngale equation ingezantsi.Cytotoxicity % = (Amanani aphilayo olawulo- Amanani aphilayo onyango) / L...

    Nge-12 ngoMeyi 30, 2020

    Imfihlo yakho ibalulekile kuthi.

    Sisebenzisa iikuki ukuphucula amava akho xa undwendwela iwebhusayithi yethu: iikuki zokusebenza zisibonisa indlela oyisebenzisa ngayo le webhusayithi, iikuki ezisebenzayo zikhumbula izinto ozikhethayo kunye neekuki ezijolise kuzo zisinceda ukuba sabelane ngomxholo ofanelekileyo kuwe.

    Yamkela

    Ngema