Countstar Mira FL

Iimpawu zemveliso

ITekhnoloji yoPhindo lwe-Innovative Optical

Itekhnoloji yokwandisa eyodwa yenza ukuba abasebenzisi bahlalutye iiseli kuluhlu olubanzi lweedayamitha

Xa usebenzisa intsimi eqaqambileyo iitemplates ze-BioApp kwi-Countstar Mira, inoveli ye-Zooming Technology yenza ukuba umqhubi akwazi ukuchonga ngokuchanekileyo izinto zeselula ngaphakathi koluhlu lwe-diamater ukusuka kwi-1.0µm ukuya kwi-180.0µm.Imifanekiso efunyenweyo ibonisa nkqu neenkcukacha zeeseli enye.Oku kwandisa uluhlu lwezicelo nakwizinto zeselula, ebezingenako ukuhlalutywa ngokuchanekileyo kwixesha elidlulileyo.

Inkqubela phambili ye-AI esekelwe kwi-algorithms yoHlalutyo lweMifanekiso

I-Countstar Mira FL isebenzisa iingenelo zeArtifical Intelligence ukuphuhlisa i-algorithms yokuzifundela.Bayakwazi ukuchonga nokuhlalutya iimpawu ezininzi zeeseli.Ukudityaniswa kweeparamitha zemilo yeeseli kuvumela uhlalutyo oluchanekileyo kunye nokuveliswa kwakhona kwimeko yomjikelezo weseli kunye / okanye ukuhambisa idatha malunga nokulungelelaniswa phakathi kokutshintsha kwe-cell morphology, ukubunjwa kwamaqela eeseli (aggregates, spheroids small sized) kunye neemeko ezichaphazelekayo.

Ukuleyibhelishwa kweziphumo zeMesenchymal Stem Cells ezimile ngendlela engaqhelekanga (MSC; 5x mangification) kwinkcubeko eyandayo

• Izangqa eziluhlaza ziphawula iiseli eziphilayo
• Izangqa ezibomvu ziphawula iiseli ezifileyo
• Isangqa ezimhlophe iiseli ezidityanisiweyo

Umgca weseli we-RAW264.7 uncinci kwaye ubambene ngokulula.I-algorithm ye-Countstar AI inokuchonga iiseli kwi-clumps kwaye ibale

• Izangqa eziluhlaza ziphawula iiseli eziphilayo
• Izangqa ezibomvu ziphawula iiseli ezifileyo
• Isangqa ezimhlophe iiseli ezidityanisiweyo

Ubungakanani obungalinganiyo beeseli ze-zebrafish embryonic (6.6X magnification

• Izangqa eziluhlaza ziphawula iiseli eziphilayo
• Izangqa ezibomvu ziphawula iiseli ezifileyo
• Isangqa ezimhlophe iiseli ezidityanisiweyo

Uyilo lwe-Intuitive Graphical User Interface (GUI).

I-GUI ecacileyo eyakhiweyo ivumela ukuphunyezwa kovavanyo olusebenzayo kunye nokhululekile

• Ithala leencwadi elibanzi elineentlobo zeseli esetwe kwangaphambili kunye ne-BioApps (iiprothokholi zetemplate zovavanyo).Ukucofa nje kube kanye kwi-BioApp, kwaye uvavanyo lunokuqala.
• I-GUI esebenziseka lula yenza kube lula ukutshintshela phakathi kweenketho ezahlukeneyo zemenyu kwaye iqinisekisa amava ovavanyo olutofotofo
• Iimodyuli ezicacileyo zemenyu ezicwangcisiweyo zixhasa umsebenzisi kuvavanyo lwemihla ngemihla

Khetha i-BioApp, faka iSampuli ye-ID, kwaye uqalise uvavanyo lwe-assay

I-128 GB yendawo yokugcina idatha yangaphakathi, eyaneleyo yokugcina i-approx.Iziphumo ze-50,000 zohlalutyo kwi-Countstar (R) Mira.Ukufikelela ngokukhawuleza, idatha efunwayo inokukhethwa ngeendlela ezahlukeneyo zokukhangela.

Uphawu oluluncedo lokonga ixesha, sisibali sokuhlambulula esifumanekayo.Iza kuhambisa imiqulu echanekileyo yesampulu yeseli edibeneyo kunye neyoqobo, nje ukuba ugxininiso lokugqibela lweeseli kunye nomthamo ekujoliswe kuwo ungenisiwe.Oku kwenza ukugqithiswa kweeseli kwi-subcultures yazo kube lula.

Iimpawu ezininzi zesicelo

Iimpawu zohlalutyo ze-Countstar Mira zixhasa umsebenzisi ekuqondeni utshintsho oluguquguqukayo ngaphakathi kwenkcubeko yeseli kwaye inceda ukunyusa iimeko zabo ezikhulayo.

Isoftware ephuculweyo, esekwe kwi-AI yokuqonda umfanekiso we-Countstar Mira iyakwazi ukuhambisa iiparamitha ezininzi.Ngaphandle kweziphumo ezisemgangathweni zokuxinaniswa kweeseli kunye nobume bokusebenza, ubungakanani bokuhanjiswa kobungakanani beeseli, ukwakheka okunokwenzeka kwamaqela eeseli, ukuqina kwe-fluorescence ehambelana neseli nganye, imo yejiko lokukhula, kunye ne-morphology factor yangaphandle ziiparamitha ezibalulekileyo zokuvavanya eyona nto ibalulekileyo. imeko yenkcubeko yeseli.Iigrafu eziveliswa ngokuzenzekelayo zokukhula kwegophe, ukusasazwa kwedayamitha kunye ne-fluorescence intensity histograms, uhlalutyo lweseli enye ngaphakathi kwee-aggregates kunye nokumiselwa kweparamitha yokuhlangana kweeseli kunceda umsebenzisi ukuba aziqonde ngcono iinkqubo eziguqukayo ngaphakathi kwenkcubeko yeseli evavanyiweyo ukusuka ekuqaleni ukuya ekupheleni kwenkqubo.

I-Histogram

I-Relative Fluorescence Intensity (RFI) yonikezelo histogram

I-histogram yokuhanjiswa kwe-diameter

Igophe lokukhula

Uvavanyo lweMifanekiso kunye neZiphumo

Umzobo wegophe lokukhula

Isicelo seMveliso

I-AO/PI i-dual fluorescence cell density kunye novavanyo lokusebenza

I-double-fluorescence AO / PI staining method isekelwe kumgaqo-siseko, ukuba zombini iidayi, i-Acridine Orange (AO) kunye nePropidium Iodide (PI), zidibanisa phakathi kwe-nucleic acids yechromosome kwinucleus yeseli.Ngelixa i-AO ikwazi ukungena kwi-membrane echanekileyo ye-nucleus nangaliphi na ixesha kwaye ingcolise i-DNA, i-PI inokudlula kuphela i-membrane edibeneyo ye-nucleus yeseli efayo (efileyo).I-AO eqokelelweyo kwi-nucleus yeseli ikhupha ukukhanya okuluhlaza kwi-525nm ephezulu, ukuba ivuyiswe yi-480nm, i-PI ithumela ukukhanya okubomvu kunye ne-amplitude yayo kwi-615nm, xa ivuya kwi-525nm.Isiphumo se-FRET (i-Foerster Resonance Energy Transfer) siqinisekisa, ukuba isignali ekhutshwayo ye-AO kwi-525nm ifunnwe phambi kwedayi ye-PI ukuphepha ukukhanya okuphindwe kabini kunye nokuchitheka.Le ndibaniselwano yedayi ekhethekileyo ye-AO/PI ivumela ukuhluza ngokukodwa i-nucleus equlethe iiseli phambi kwee-acaryotes ezifana ne-erythrocytes.

Idatha ye-Countstar Mira FL ibonise umgca olungileyo wokuhlanjululwa kwe-gradient yeeseli ze-HEK293

Uhlalutyo lwe-GFP/RFP lokugqithiselwa kakuhle

Ukusebenza kakuhle kodluliselo sisalathiso esibalulekileyo ekuphuhliseni umgca weseli kunye nokulungelelaniswa, kwi-viral vector tuning, kunye nokubeka iliso kwimveliso kwiinkqubo zeBiopharma.Ibe lolona vavanyo luxhaphakileyo ukumisela ngokukhawuleza ngokuthembekileyo umxholo weprotheyini ekujoliswe kuyo ngaphakathi kwiseli.Kwiindlela ezahlukeneyo zonyango lwemfuza, sisixhobo esiyimfuneko sokulawula ukosuleleka kotshintsho lwemfuzo olufunekayo.

I-Countstar Mira ayinikezeli kuphela iziphumo ezichanekileyo nezichanekileyo, xa kuthelekiswa nokuhamba kwe-cytometry, ukongezelela i-analyzer inikeza imifanekiso njengobungqina bobungqina.Ngaphandle koku, ilula kakhulu kwaye ikhawulezise uhlalutyo ukuze kulungiswe uphuhliso lwenkqubo yophuhliso kunye nemveliso.

Uchungechunge lwemifanekiso, efunyenwe yi-Countstar (R) Mira, ebonisa ukunyuka kwamanqanaba okusebenza kakuhle (ukusuka kwesobunxele ukuya kwesokudla) kweeseli eziguqulwe ngofuzo (HEK 293 umgca weseli; ukuvakalisa i-GFP kwiindawo ezahlukeneyo)

Iziphumo zemilinganiselo yokuthelekisa, eyenziwa nge-B / C CytoFLEX, eqinisekisa idatha ye-GFP yokudluliselwa kwe-GFP yeeseli eziguquliweyo ze-HEK 293, ezihlalutywe kwi-Countstar Mira.

Uhlalutyo olusekwe ngokubanzi lweTrypan Blue

Uvavanyo localucalulo lwe-Trypan Blue luseyeyona ndlela isetyenziswa kakhulu nethembekileyo yokumisela inani (ezifayo) iiseli ezifileyo ngaphakathi kwenkcubeko yeseli yokunqunyanyiswa.Iiseli ezisebenzayo ezinolwakhiwo lwemembrane yeseli yangaphandle ziya kuyigxotha i-Trypan Blue ekubeni igqobhoze kwi-membrane.Kwimeko apho, i-membrane yeseli iyavuza ngenxa yenkqubela phambili yokufa kweeseli zayo, i-Trypan Blue inokudlula umqobo we-membrane, iqokelele kwiplasma yeseli kwaye ingcolise iseli eluhlaza.Lo mahluko wamehlo ungasetyenziselwa ukwahlula iiseli eziphilayo ngokungenasiphelo kwiiseli ezifileyo ngomfanekiso wokuqatshelwa kwe-algorithms ye-Countstar Mira FL.

• Imifanekiso emithathu, i-Trypan Blue imigca yeseli enemibala, efunyenwe kwi-Countstar (R) i-Mira FL kwimodi yentsimi eqaqambileyo.

• Iziphumo zedilution ye-HEK 293 series