Igazi namaseli ayinhloko asanda kuhlukaniswa noma amaseli athuthukisiwe angase aqukathe ukungcola, izinhlobo ezimbalwa zamaseli noma izinhlayiya eziphazamisayo ezifana nemfucumfucu yamaseli okuzokwenza kungenzeki ukuhlaziya amaseli athakaselwayo.I-Countstar FL enohlaziyo lwendlela ye-fluorescence ekabili ingase ikhiphe izingcezwana zamaseli, udoti nama-artifacts izinhlayiya kanye nezenzakalo ezingaphansi kwama-platelet, okunikeza umphumela onembe kakhulu.
Ukubala kokusebenza kwe-AO/PI Dual Fluorescence
I-Acridine orange (AO) nePropidium iodide (PI) amadayi abopha i-nucleic acid.Ukuhlaziya akubandakanyi izingcezu zamaseli, udoti kanye nezinhlayiya ze-artifact kanye nezehlakalo ezincane ezifana namaseli abomvu egazi, okunikeza umphumela onembe kakhulu.Sengiphetha, uhlelo lwe-Countstar lungasetshenziswa kuso sonke isinyathelo senqubo yokukhiqiza amaseli.
Ama-WBC Egazini Eliphelele
Umfanekiso 2 Isithombe esiphelele sesampula segazi esithathwe u-Countstar Rigel
Ukuhlaziya ama-WBC egazini lonke kuwukuhlola okuvamile elebhu yomtholampilo noma endaweni yokugcina igazi.Ukugxila nokusebenza kwama-WBCs kuyinkomba ebalulekile njengokulawulwa kwekhwalithi yokugcinwa kwegazi.
I-Countstar Rigel enendlela ye-AO/PI ingahlukanisa ngokunembile isimo esiphilayo nesifile samaseli.I-Rigel ingakwazi futhi ukubala i-WBC ngokunembile kuyilapho ingafaki ukuphazamiseka kwamaseli abomvu egazi.
Ukubala kanye nokusebenza kwe-PBMC
Umfanekiso we-3 Bright Field kanye nezithombe ze-Fluorescence ze-PBMC Zithathwe ngu-Countstar Rigel
Ukubala kwe-AOPI Dual-fluoresces wuhlobo lokuhlola olusetshenziselwa ukuthola ukugcwala kwamaseli nokusebenza.Ngenxa yalokho, amaseli angama-nucleated anolwelwesi olungaguquki angcolisa okuluhlaza okotshani futhi abalwa njengaphilayo, kuyilapho amaseli e-nucleated anolwelwesi olusengozini angcolisa kuphela okubomvu kwe-fluorescent futhi abalwa njengafile lapho kusetshenziswa uhlelo lwe-Countstar Rigel.Izinto ezingezona i-nucleated njengamaseli abomvu egazi, ama-platelet kanye nemfucumfucu akushintshi futhi kuzitshwa isofthiwe ye-Countstar Rigel.
