Isampula eliqukethe amaseli amisiwe lixutshwa nodayi oluhlaza we-Trypan, bese lidonswa ku-Countstar Chamber Slide esihlaziywa yi-Countstar Automated Cell Counter.Ngokusekelwe kumgomo wakudala wokubala kwamaseli e-trypan eluhlaza okwesibhakabhaka, amathuluzi e-Countstar ahlanganisa ubuchwepheshe obuthuthukisiwe be-optical imaging, ubuchwepheshe obuhlakaniphile bokuqaphela isithombe kanye nama-algorithms esofthiwe anamandla ukuze anganikezi nje kuphela ukugxila kweseli nokusebenza, kodwa futhi anikeze ulwazi lokugxiliswa kweseli, ukusebenza, izinga lokuhlanganisa, ukujikeleza. , nokusabalalisa kobubanzi ngokugijima okukodwa kuphela.
Ukuhlaziywa Kweseli Elihlanganisiwe
Umfanekiso 3 Ukubalwa kwamaseli ahlanganisiwe.
A. Isithombe Sesampula Seseli;
B. Isithombe Sesampuli Yeseli enophawu lokuhlonza ngesofthiwe ye-Countstar BioTech.(Umjikelezo Oluhlaza: Iseli elibukhoma, Umbuthano Ophuzi: Iseli elifile, Umbuthano Obomvu: Iseli Elihlanganisiwe).
C. I-Histogram Ehlanganisiwe
Amanye amaseli ayisisekelo noma amaseli e-subculture athambekele ekubeni ahlanganiswe lapho isiko elimpofu lisesimweni esibi noma ukugayeka kokudla ukudla ngokweqile, ngaleyo ndlela kubangele ubunzima obukhulu ekubaleni amaseli.Ngomsebenzi Wokulinganisa Ukuhlanganisa, i-Countstar ingathola isibalo esishukumisayo sokuhlanganisa ukuze kuqinisekiswe ukubalwa kwamaseli okunembile futhi ithole izinga lokuhlanganisa kanye ne-histogram yokuhlanganisa, ngaleyo ndlela inikeze isisekelo sabahloli ukuze bahlulele isimo samaseli.
Ukuqapha Ukukhula Kweseli
Umfanekiso we-4 Cell Grow Curve.
Ijika lokukhula kweseli iyindlela evamile yokulinganisa ukukhula okuphelele kwenombolo yeseli, inkomba ebalulekile yokunquma ukugcwala kwamaseli kanye nemingcele eyisisekelo yesiko lezinto eziyisisekelo zebhayoloji zamaseli.Ukuze uchaze ngokunembile uguquko oluguquguqukayo enanini lamaseli kuyo yonke inqubo, ijika elivamile lokukhula lingahlukaniswa libe izingxenye ezingu-4: isikhathi sokufukamela ngokukhula kancane;isigaba sokukhula esine-slope enkulu, isigaba sethafa kanye nesikhathi sokwehla.Ijika lokukhula kweseli lingatholwa ngokuhlela inani lamaseli aphilayo (10'000/mL) ngokumelene nesikhathi samasiko (h noma d).
Ukulinganisa Ukugxila Kweseli nokusebenza
Umfanekiso 1 Izithombe zithwetshulwe yi-Countstar BioTech njengamaseli (i-Vero, i-3T3, i-549, i-B16, i-CHO, i-Hela, i-SF9, ne-MDCK) imisiwe yangcoliswa i-Trypan Blue ngokulandelanayo.
I-Countstar isetshenziswa kumaseli anobubanzi obuphakathi kuka-5-180um, njengeseli elincelisayo, iseli lezinambuzane, namapulangwe athile.
Ukukala Usayizi Weseli
Umfanekiso 2 Ukulinganisa Usayizi Weseli wamaseli e-CHO ngaphambi nangemva kokudluliselwa kwe-plasmid.
A. Izithombe zokumiswa kwamaseli e-CHO ezingcoliswe i-trypan eluhlaza okwesibhakabhaka ngaphambi nangemuva kokudluliselwa kwe-plasmid.
B. Ukuqhathaniswa kwe-CHO cell size histogram ngaphambi nangemva kokudluliswa kwe-plasmid.
Ukushintsha kosayizi weseli kuyisici esibalulekile futhi kuvame ukukalwa ocwaningweni lwamaseli.Ngokuvamile izokalwa kulokhu kuhlolwa: ukudluliswa kweseli, ukuhlolwa kwezidakamizwa kanye nokuhlolwa kokusebenza kweseli.I-Countstar inikeza idatha yezibalo ye-morphology, efana nosayizi bamaseli, phakathi kwama-20s.
I-Countstar automated cell counter inganikeza idatha ye-morphological yamaseli, okuhlanganisa ukujikeleza kanye nama-histograms obubanzi.
