Ukwelashwa ngamaseli ngokungangabazeki kuyithemba elisha lokuhola ikusasa le-biomedicine, kodwa ukusetshenziswa kwamangqamuzana omuntu kwezokwelapha akuwona umqondo omusha.Emashumini ambalwa eminyaka adlule, ukwelashwa kwamangqamuzana kuye kwathuthuka kakhulu, futhi ukwelashwa kwamangqamuzana ngokwako akusaselona iqoqo elilula lamaseli futhi libuyiselwa emuva.Amaseli manje avame ukudingeka ukuze enziwe nge-bioengineered, njengokwelashwa kwamaseli e-CAR-T.Sihlose ukukuhlinzeka ngezisetshenziswa ezisezingeni le-GMP zokulawula ikhwalithi yamaseli.Umkhiqizo we-Countstar wamukelwe yizinkampani eziningi ezihola ekwelashweni kwamaseli, singasiza ikhasimende lethu ukuthi lakhe ukugxiliswa kwamaseli okuzinzile, okuthembekile, uhlelo lokuqapha ukusebenza kahle.
Inselele ekubalweni kwamaseli nokusebenza
Phakathi kwazo zonke izinyathelo zokukhiqiza amaseli e-CAR-T yomtholampilo, ukusebenza nokubala kwamaseli kufanele kunqunywe ngokunembile.
Amaseli ayinhloko asanda kuhlukaniswa noma amaseli akhulisiwe angase aqukathe ukungcola, izinhlobo ezimbalwa zamaseli noma izinhlayiya eziphazamisayo ezifana nemfucumfucu yamaseli okuzokwenza kungenzeki ukuhlaziya amaseli athakaselwayo.
Ukubalwa kokusebenza kwe-Dual Fluorescence ngu-Countstar Rigel S2
I-Acridine orange (AO) nePropidium iodide (PI) amadayi abopha i-nucleic acid.I-AO ingangena kuwo womabili amaseli afile naphilayo futhi ingcolise amaseli ane-nucleated ukuze ikhiqize i-fluorescence eluhlaza.I-PI ingangcolisa amaseli ayi-nucleated afile ngolwelwesi olusengozini futhi ikhiqize i-fluorescence ebomvu.Ukuhlaziya akubandakanyi izingcezu zamaseli, udoti nama-artifacts izinhlayiya kanye nezehlakalo ezingaphansi kwama-platelet, okunikeza umphumela onembe kakhulu.Sengiphetha, uhlelo lwe-Countstar S2 lungasetshenziswa kuso sonke isinyathelo senqubo yokukhiqiza amaseli.
A: Indlela ye-AO/PI ingahlukanisa ngokunembile isimo esiphilayo nesifile samaseli, futhi ingafaka ngaphandle ukuphazamiseka.Ngokuhlola amasampula wokuxubha, indlela ye-dual-fluorescence ibonisa imiphumela ezinzile.
Ukunqunywa kwe-T/NK Cell Mediated Cytotoxicity
Ngokulebula amaseli wesimila okuhlosiwe ngawo nge-calcein AM engenabuthi, engeyona imisebe noma i-transfect nge-GFP, singaqapha ukubulawa kwamaseli wesimila ngamaseli e-CAR-T.Ngenkathi amangqamuzana omdlavuza oqondiswe bukhoma azobhalwa nge-calcein eluhlaza i-AM noma i-GFP, amaseli afile awakwazi ukugcina idayi eluhlaza.I-Hoechst 33342 isetshenziselwa ukungcolisa wonke amaseli (womabili amaseli e-T namaseli wesimila), esikhundleni salokho, amaseli wesimila okuhlosiwe angangcoliswa ngolwelwesi oluboshwe i-calcein AM, i-PI isetshenziselwa ukungcolisa amaseli afile (womabili amaseli T namaseli isimila).Lelisu lokungcolisa livumela ukucwaswa kwamaseli ahlukene.
Ukubalwa kwamaseli okungaguquki kanye nokuphathwa kwedatha yomhlaba wonke
Inkinga evamile ekubaleni amaseli okuvamile umehluko wedatha phakathi kwabasebenzisi, iminyango namasayithi.Wonke umhlaziyi we-Countstar ubala okufanayo endaweni ehlukile noma indawo yokukhiqiza.Lokhu kungenxa yokuthi ohlelweni lokulawula ikhwalithi, insimbi ngayinye kufanele ilinganiswe ethuluzini elijwayelekile.
Ibhange ledatha elimaphakathi livumela umsebenzisi ukuthi agcine yonke idatha, njengombiko wokuhlolwa kwensimbi, umbiko wesampula yeselula nesiginesha ye-e yomhloli, iphephile futhi unomphela.
I-Car T Cell Therapy: Ithemba Elisha Lokwelashwa Komdlavuza
I-CAR-T cell therapy ngokungangabazeki iyithemba elisha ekuholeni ikusasa le-biomedicine yomdlavuza.Phakathi kwazo zonke izinyathelo zokukhiqiza amaseli e-CAR-T yomtholampilo, ukusebenza nokubala kwamaseli kufanele kunqunywe ngokunembile.
I-Countstar Rigel yamukelwe izinkampani eziningi ezihola i-CAR-T cell therapy, singakwazi ukusiza ikhasimende lethu ukuthi lakhe uhlelo lokugxilisa amaseli oluzinzile, oluthembekile, uhlelo lokuqapha ukusebenza kahle.
