I-Trypan Blue Cell Counting
Ukuqapha nokuhlaziya isiko lamaseli ngezixazululo zesimanjemanje.Ukuqapha okuthembekile nokusebenzayo kubalulekile ekuthuthukiseni umkhiqizo kanye nekhwalithi yomkhiqizo njengoba ngisho nezinguquko ezincane kumapharamitha we-bioprocess zingathonya ukusebenza kwesiko leseli lakho.Ukubalwa kwamaseli nokusebenza kwayo kuyimingcele ebaluleke kakhulu, i-Countstar® Altair inikeza isixazululo esihlakaniphe ngokwedlulele nesithobelana ngokugcwele nesixazululo se-cGMP salokhu.
Ngesikhathi se-bioprocess, i-GFP ivame ukusetshenziselwa ukuhlanganisa amaprotheni ahlangene njengenkomba.Nquma i-GFP fluorescent ingabonisa i-protein expression.I-Countstar Rigel inikezela ngesivivinyo esisheshayo nesilula sokuhlola ukudluliselwa kwe-GFP kanye nokusebenza.Amaseli agcotshwe nge-Propidium iodide (PI) kanye ne-Hoechst 33342 ukuze achaze inani lamaseli afile kanye nengqikithi yamaseli.I-Countstar Rigel inikezela ngendlela esheshayo, yobuningi yokuhlola ukusebenza kahle kokuvezwa kwe-GFP nokusebenza ngesikhathi esifanayo.
Sisebenzisa amakhukhi ukuthuthukisa ulwazi lwakho lapho uvakashela amawebhusayithi ethu: amakhukhi okusebenza asibonisa ukuthi uyisebenzisa kanjani le webhusayithi, amakhukhi asebenzayo akhumbula okuncamelayo futhi amakhukhi aqondisayo asisiza ukuthi sabelane ngokuqukethwe okuhlobene nawe.