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I-Countstar BioFerm

I-Countstar automated fungi suspension cell analyzer

I-Countstar BioFerm automated fungi cell analyzer ihlanganisa izindlela zakudala zokungcolisa kusetshenziswa i-Methylene Blue, i-Trypan Blue, i-Methylene Violet, noma i-Erythrosin B ngesithombe sokulungiswa okuphezulu.Ama-algorithms okuqaphela ukuhlaziya izithombe aletha ukutholakala okunembayo nokunembile kwamaseli esikhunta asebenzayo nafile, ukugcwala kwawo kwamaseli, ububanzi kanye nolwazi mayelana ne-morphology kanye.Uhlelo olunamandla lokuphatha idatha lulondoloza ngokuthembekile imiphumela nezithombe futhi luvumela ukuhlaziya kabusha nganoma yisiphi isikhathi.  

 

Ibanga Lohlelo

I-Countstar BioFerm iyakwazi ukubala futhi ihlaziye izinhlobo eziningi zesikhunta (kanye nezihlanganisi zazo) kububanzi obuphakathi kuka-2μm kuya ku-180μm.Embonini ye-biofuel kanye ne-biopharma, i-Countstar BioFerm ifakazele amandla ayo njengethuluzi elithembekile nelisheshayo lokuqapha izinqubo zokukhiqiza.

 

Izinzuzo Zomsebenzisi

  • Ulwazi oluphelele mayelana nesikhunta
    Idatha ihlanganisa ulwazi mayelana nokugxilisa ingqondo, ukusebenza, ububanzi, ukubumbana, nezinga lokuhlanganisa.
  • I-“Fixed Focus Technology” yethu enelungelo lobunikazi
    Asikho isidingo noma nini ukulungisa ukugxila kwe-Countstar BioFerm.
  • Ibhentshi elibonakalayo elinekhamera enemibala engu-5-megapixel
    Iqinisekisa ukubona ngeso okucebile nokunemininingwane yezinto eziphilayo.
  • Imojuli yokuhlaziya yokuhlanganisa
    Ivumela isitatimende esithembekile mayelana nomsebenzi osafufusa
  • Izinto ezisetshenziswayo ezonga izindleko
    Izikhundla eziyisampula ezinhlanu kusilayidi esisodwa se-Countstar Chamber kunciphisa izindleko zokusebenza, udoti wepulasitiki, futhi konga isikhathi sokuhlola.
  • Imininingwane yomkhiqizo
  • Imininingwane Yezobuchwepheshe
  • Landa
Imininingwane yomkhiqizo

 

 

Izithombe Ezisampula Ze-Baker's Yeast Saccharomyces cerevisiae

 

Izithombe zemvubelo yokubhaka I-Saccharomyces cerevisiae itholwe nge-Countstar BioFerm. Amasampula athathwe ezinqubweni ezihlukene zokukhiqiza, ingxenye egcotshwe nge-Methylene Blue (phansi kwesokunxele) kanye ne-Methylene Violet (kwesokudla ngezansi)

 

 

 

I-Saccharomyces cerevisiae ezigabeni ezihlukene zenqubo yokuvutshelwa eyizinyathelo ezimbili

 

Phezulu kwesokunxele: Ingxenye yesithombe se-Countstar BioFerm esibonisa isiko lokuthoma, esingcoliswe i-Methylene Blue (MB).Isampula iqukethe ukuminyana kwamaseli aphezulu futhi amaseli ayasebenza kakhulu (ukufa okulinganiselwe <5%).Ngaphansi kwesokunxele: Isampula elingagcotshiwe elivela ku-bioreactor esanda kugotshwa;amahlumela abonakala ngokucacile.Ngezansi kwesokudla: Isampula lithathwe esigabeni sokugcina senqubo yokuvubela eyinhloko, engcoliswe ngu-1:1 nge-MB (isilinganiso sokufa okulinganiselwe: 25%).Imicibisholo ebomvu imaka amaseli afile, ahlanganisa udayi osebenzayo we-MB, okuholela kumbala omnyama wawo wonke umthamo weseli.

 

 

 

Ukuqhathaniswa kwedatha yokulinganisa

 

Ihluzo ezingenhla zibonisa ukuqhathaniswa kwe-Countstar BioFerm nokubala okwenziwa ngesandla, kanye nokwehluka okubalulekile okuphansi emiphumeleni yokulinganisa, uma kuqhathaniswa nezibalo ze-hemocytometer ezenziwa ngesandla.

 

Ukuqhathaniswa kokuhlaziywa kokusabalalisa kobubanzi okwenziwa ngesandla nokuzenzakalelayo

 

 

Izithombe ezingenhla zibonisa ukunemba okuphezulu kwezilinganiso zobubanzi be-Countstar BioFerm kunhlolovo eyenziwa ngesandla ku-hemocytometer.Njengoba kubhukwana libala inani eliphansi eliphindwe izikhathi eziyi-100 lamaseli liyahlaziywa, iphethini yokusabalalisa ububanzi iyahluka kakhulu kune-Countstar BioFerm, lapho kwahlaziywa khona amaseli eyeast acishe abe ngu-3,000.

 

 

 

Ukuphindaphindeka kokubalwa kwamaseli nezinga lokufa

 

25 ama-aliquots ahlanjululwe I-Saccharomyces cerevisiae amasampula, aqukethe ukugxila okuzisholo kwamaseli angu-6.6×106/mL ahlaziywa ngokuhambisana yi-Countstar BioFerm kanye ne-hemocytometer ngesandla.

Zombili ihluzo zibonisa ukuhluka okuphezulu kakhulu ekubalweni kweseli elilodwa, okwenziwa mathupha ku-hemocytometer.Ngokuphambene, i-Countstar BioFerm ihluka kancane kuphela enanini legama lokugxilisa ingqondo (kwesokunxele) nokufa (kwesokudla).

 

I-Saccharomyces cerevisiae ezigabeni ezihlukene zenqubo yokuvutshelwa eyizinyathelo ezimbili

 

I-Saccharomyces cerevisiae, ingcoliswe yi-Methylene Violet futhi kamuva yahlaziywa nge-Countstar Isistimu ye-BioFerm

Kwesokunxele: Ingxenye yesithombe esitholiwe se-Countstar Bioferm Kwesokudla: Isigaba esifanayo, amaseli abhalwe i-Countstar Ama-algorithms wokubona isithombe se-BioFerm.Amaseli asebenzayo azungezwe yindilinga eluhlaza, amaseli anamabala (afile). ephawulwe ngezindilinga eziphuzi (ngokwengeziwe kukhonjiswe le ncwajana enemicibisholo ephuzi).Kuhlanganisiwe amaseli azungezwe yizindilinga ezibomvana.Inani eliphakeme lama-cell aggregate amabili liyabonakala - inkomba ecacile yomsebenzi okhulayo walesi siko, imicibisholo ephuzi, efakwe ngesandla, maka amaseli afile.

 

I-histogram ehlanganisiwe yokuvutshelwa kweyeast ekhula ngamandla ibhala izinga eliphezulu lomsebenzi wokuhluma, ebonisa ikakhulukazi amangqamuzana amaseli angu-2,

Imininingwane Yezobuchwepheshe

 

 

Imininingwane Yezobuchwepheshe
Okukhiphayo Kwedatha Ukugxila, Ukufa, Ububanzi, Izinga Lokuhlanganisa, Ukuqina
Ibanga Lokulinganisa 5.0 x 10 4 - 5.0 x 10 7 /ml
Ububanzi besayizi 2 – 180 μm
Ivolumu yeChamber 20 ml
Isikhathi Sokulinganisa <20 amasekhondi
Ifomethi Yomphumela Isipredishithi se-JPEG/PDF/Excel
Okokusebenza 5 Amasampula / Countstar Chamber Slide

 

 

Imininingwane Yesilayidi
Okubalulekile I-Poly-(Methyl) Methacrylate (PMMA)
Ubukhulu: 75 mm (w) x 25 mm (d) x 1.8 mm (h)
Ukujula Kwegumbi: 190 ± 3 μm (kuphela ngo-1.6% ukuchezuka ngobude ngokunemba okuphezulu)
Ivolumu yeChamber 20 ml

 

 

Landa
  • I-Countstar BioFerm Brochure.pdf Landa

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