Ukusebenzisa I-Cytometer Yesithombe Ukuze Kuthuthukiswe Ukuthuthukiswa Kolayini Bamaseli we-Biologics kanye nokukhiqizwa kwe-rAAV

I-Biologics kanye ne-AAV-based gene therapy zithola isabelo semakethe esengeziwe sokwelashwa kwezifo.Kodwa-ke, ukwakha ulayini wamaseli wezilwane ezincelisayo oqinile futhi osebenzayo ukuze akhiqize kuyinselele futhi ngokuvamile kudinga ukuhlukaniswa okubanzi kwamangqamuzana.Ngokomlando, i-cytometer egelezayo isetshenziswa kulezi zivivinyo ezisekelwe kumaseli.Kodwa-ke, i-cytometer yokugeleza ibiza kakhulu futhi ihilela ukuqeqeshwa okubanzi kokubili ukusebenza nokugcinwa.Muva nje, ngokukhula kwamandla ekhompuyutha nezinzwa zekhamera zekhwalithi ephezulu, i-cytometry esekelwe ezithombeni iye yasungulwa ukuze inikeze enye indlela enembile nengabizi kakhulu yokuthuthukiswa kwenqubo yomugqa weselula.Kulo msebenzi, sichaze ukugeleza komsebenzi wokuthuthukisa ulayini weselula ohlanganisa i-cytometer esekwe esithombeni, okuyi-Countstar Rigel, yokuhlola ukusebenza kahle kokudluliselwa kwegciwane kanye nokuhlolwa kwechibi okuzinzile kusetshenziswa amaseli e-CHO kanye ne-HEK293 aveza i-antibody kanye ne-rAAV vector, ngokulandelana.Kulezi zifundo ezimbili, sibonise:

1. I-Countstar Rigel inikeze ukunemba okufanayo kokutholwa ku-cytometry egelezayo.
2. Ukuhlola kwechibi okusekelwe ku-Countstar Rigel kungasiza ekunqumeni iqembu elifiselekayo le-single-cell cloning (SCC).
3. I-Countstar Rigel ihlanganise inkundla yokuthuthukisa ulayini wamaselula izuze u-2.5 g/L mAb titer.

Siphinde saxoxa ngamathuba okusebenzisa i-Countstar njengolunye ungqimba lwethagethi yokuthuthukisa esekelwe ku-rAAV DoE.

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