Ukuhlaziywa kwe-Immuno-phenotyping kuwukuhlola okuvamile okwenziwa emikhakheni yocwaningo ehlobene namaseli ukuze kuhlonzwe izifo ezihlukahlukene (isifo esizimele, isifo sokuzivikela komzimba, isifo sokuxilongwa kwesimila, ukopha, izifo ezibangelwa yi-allergic, nokunye okuningi) kanye ne-pathology yezifo.Ibuye isetshenziselwe ukuhlola ikhwalithi yeseli ocwaningweni lwezifo zamaseli ezinhlobonhlobo.I-Flow cytometry kanye ne-fluorescence microscope izindlela zokuhlaziya ezijwayelekile ezikhungweni zocwaningo lwezifo zamaseli ezisetshenziselwa i-immuno-phenotyping.Kodwa lezi zindlela zokuhlaziya zinganikeza izithombe noma uchungechunge lwedatha, kuphela, okungenzeka zingahlangabezani nezimfuneko zokugunyazwa eziqinile zeziphathimandla ezilawulayo.
M Dominici el, Cytotherapy (2006) Umq.8, No. 4, 315-317
Ukuhlonzwa kwe-Immuno-phenotype ye-AdMSCs
I-immunophenotype ye-AdMSCs yanqunywa i-Countstar FL, i-AdMSCs yafakwa emasosheni amasosha omzimba ahlukene ngokulandelana (CD29, CD34, CD45, CD56, CD73, CD105, kanye ne-HLADR).Inqubo yokufaka umbala wesignali idalwe ngokusethwa kweshaneli eluhlaza ukuze kufanekisele i-PE fluorescence, kanye nenkambu ekhanyayo.Isegimenti yereferensi yenkundla ekhanyayo isetshenziswe njengemaski ukuze kusampula isignali ye-PE fluorescence.Imiphumela ye-CD105 ikhonjisiwe (Umfanekiso 1).
Umfanekiso 1 Ukuhlonzwa kwe-Immuno-phenotype ye-AdMSCs.A. Inkundla Ekhanyayo kanye nesithombe se-Fluorescence sama-AdMSC;B. Ukutholwa Umaka we-CD wama-AdMSC ngu-Countstar FL
Ukulawulwa kwekhwalithi yama-MSC - imiphumela eqinisekisayo yeseli ngalinye
Umfanekiso 2 A: Imiphumela ye-Countstar FL iboniswe ku-FCS express 5plus, ithola iphesenti elihle le-CD105, kanye nohlolojikelele lwamaseli ethebula elilodwa.B: Isango lokungena elilungisiwe ohlangothini lwesokudla, izithombe zethebula leseli elilodwa libonisa lawo maseli anenkulumo ephezulu ye-CD105.C: Isango lokungena elilungisiwe ohlangothini lwesobunxele, izithombe zethebula leseli elilodwa libonisa lawo maseli anenkulumo ephansi ye-CD105.
Izinguquko Ze-Phenotypical phakathi Nezokuthutha
Umfanekiso 3. A: Ukuhlaziywa komthamo wephesenti elihle le-CD105 kumasampuli ahlukene nge-software ye-FCS express 5 plus.B: Izithombe zekhwalithi ephezulu zinikeza ulwazi olwengeziwe lwe-morphological.C: Imiphumela eqinisekisiwe ngezithonjana zeseli ngayinye, amathuluzi esofthiwe ye-FCS ahlukanisa amaseli aba ahlukene
