Isingeniso
Ukulinganisa ukufakwa kodayi ababopha i-DNA kube yindlela esungulwe kahle yokunquma okuqukethwe kwe-DNA yeselula ekuhlaziyweni komjikelezo weseli.I-Propidium iodide (PI) udayi onamabala we-nuclear ovame ukusetshenziswa ekulinganiseni umjikelezo weseli.Ekuhlukaneni kwamaseli, amaseli aqukethe amanani anyukile e-DNA abonisa ngokulinganayo ukwanda kwe-fluorescence.Umehluko kumfutho we-fluorescence usetshenziselwa ukunquma okuqukethwe kwe-DNA esigabeni ngasinye somjikelezo weseli.I-Countstar Rigel system (Fig.1) iyithuluzi lokuhlaziya amangqamuzana elihlakaniphile, elinembile, elinemisebenzi eminingi elingathola idatha enembile ekuhlaziyeni umjikelezo wamaseli futhi lingathola i-cytotoxicity ngokuhlolwa kokusebenza kwamaseli.Inqubo esebenziseka kalula, ezenzakalelayo ikuqondisa ukuthi uqedele ukuhlola kwamaselula kusukela ekuthwebuleni nasekutholeni idatha.
