I-Experimental Protocol
I-cytotoxicity % ibalwa ngesibalo esingezansi.
I-Cytotoxicity % = (Izibalo ezibukhoma zokulawula - Izibalo ezibukhoma zokwelashwa) / Izibalo ezibukhoma zokulawula × 100
Ngokulebula amaseli wesimila okuhlosiwe ngawo nge-calcein AM engenabuthi, engeyona imisebe noma i-transfect nge-GFP, singaqapha ukubulawa kwamaseli wesimila ngamaseli e-CAR-T.Ngenkathi amangqamuzana omdlavuza oqondiswe bukhoma azobhalwa nge-calcein eluhlaza i-AM noma i-GFP, amaseli afile awakwazi ukugcina idayi eluhlaza.I-Hoechst 33342 isetshenziselwa ukungcolisa wonke amaseli (womabili amaseli e-T namaseli wesimila), esikhundleni salokho, amaseli wesimila okuhlosiwe angangcoliswa ngolwelwesi oluboshwe i-calcein AM, i-PI isetshenziselwa ukungcolisa amaseli afile (womabili amaseli T namaseli isimila).Lelisu lokungcolisa livumela ukucwaswa kwamaseli ahlukene.
E: I-T Ratio encike ku-Cytotoxicity ye-K562
Isibonelo se-Hoechst 33342, CFSE, PI fluorescent izithombe amaseli aqondiwe we-K562 ku-t = amahora angu-3
Izithombe eziba umphumela ze-fluorescent zibonise ukwenyuka kwamaseli e-Hoechst+CFSE+PI+ Target njengoba u-E: T isilinganiso senyuka
